Abstract:
Mycoviruses are known to be difficult to cure in fungi but their spontaneous loss occurs
commonly. The unexpected disappearance of mycoviruses can be explained by diverse reasons,
from methodological procedures to biological events such as posttranscriptional silencing machinery.
The long-term effects of a virus infection on the host organism have been well studied in the case of
human viruses; however, the possible residual effect on a fungus after the degradation of a mycovirus
is unknown. For that, this study analyses a possible residual effect on the transcriptome of the
pathogenic fungus Fusarium circinatum after the loss of the mitovirus FcMV1. The mycovirus that
previously infected the fungal isolate was not recovered after a 4-year storage period. Only 14 genes
were determined as differentially expressed and were related to cell cycle regulation and amino acid
metabolism. The results showed a slight acceleration in the metabolism of the host that had lost the
mycovirus by the upregulation of the genes involved in essential functions for fungal development.
The analysis also revealed a weak expression in the annotated genes of the RNA silencing machinery.
To our knowledge, this is the first time that a potential residual effect on the host transcriptome
caused by the past infection of a mycovirus is reported.
Description:
SUPPORTING INFORMATION : Figure S1: Venn diagram of the differentially expressed genes by the post-infection of the virus FcMV1. Figure S2: Sequences of the differentially expressed genes by the post-infection of the virus FcMV1 without a known function. Figure S3: Scatter plot representing the differentially expressed genes of this analysis. Xaxis: normalized means in condition free of FcMV1 infection. Y-axis: normalized means in condition of FcMV1 post-infection. Red dot: downregulated genes; green dot: upregulated genes. Figure S4: FET enriched GO terms associated with the differentially expressed genes of Fusarium circinatum previously infected by FcMV1. Figure S5: Distribution of GO terms represented in this analysis by biological processes, cellular components and molecular functions. Figure S6: Distribution of the sequences of the analysis in the InterProScan domains. Figure S7: Blast2GO blast results of the highest similarity species with higher Max ident and lower evalues. Table S1: RNA sequencing and genome mapping statistics. Table S2: Genes highly expressed of F. circinatum shared in both conditions. Table S3: FET enriched GO terms associated with the genes highly expressed of F. circinatum shared in both conditions. Table S4: Genes highly expressed in Fc072v cultures of F. circinatum. Table S5: FET enriched GO terms associated with the genes highly expressed in Fc072v cultures of F. circinatum. Table S6: Genes highly expressed in Fc072 cultures of F. circinatum. Table S7: FET enriched GO terms associated with the genes upregulated in F. circinatum by the FcMV1 residual effect. Table S8: Blast homology result of the genes implicated in post transcriptional gene silencing in Fusarium circinatum. Table S9: Expression ratios (RPKM and fold-change relative to the virus-free monosporic) of Fusarium circinatum genes involved in post transcriptional gene silencing.
