dc.contributor.author |
Bezza, Fisseha Andualem
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|
dc.contributor.author |
Tichapondwa, Shepherd Masimba
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|
dc.contributor.author |
Chirwa, Evans M.N.
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dc.date.accessioned |
2021-07-28T13:05:31Z |
|
dc.date.available |
2021-07-28T13:05:31Z |
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dc.date.issued |
2020-10-07 |
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dc.description.abstract |
Cuprous oxide nanoparticles (Cu2O NPs) were fabricated in reverse micellar templates by using lipopeptidal biosurfactant as a stabilizing agent. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy dispersive x-ray spectrum (EDX) and UV–Vis analysis were carried out to investigate the morphology, size, composition and stability of the nanoparticles synthesized. The antibacterial activity of the as-synthesized C u2O NPs was evaluated against Gram-positive B. subtilis CN2 and Gram-negative P. aeruginosa CB1 strains, based on cell viability, zone of inhibition and minimal inhibitory concentration (MIC) indices. The lipopeptide stabilized Cu2O NPs with an ultrasmall size of 30 ± 2 nm diameter exhibited potent antimicrobial activity against both Gram-positive and Gram-negative bacteria with a minimum inhibitory concentration of 62.5 μg/mL at pH5. MTT cell viability assay displayed a median inhibition concentration ( IC50) of 21.21 μg/L and 18.65 μg/mL for P. aeruginosa and B. subtilis strains respectively. Flow cytometric quantification of intracellular reactive oxygen species (ROS) using 2,7-dichlorodihydrofluorescein diacetate staining revealed a significant ROS generation up to 2.6 to 3.2-fold increase in the cells treated with 62.5 μg/mL Cu2O NPs compared to the untreated controls, demonstrating robust antibacterial activity. The results suggest that lipopeptide biosurfactant stabilized C u2O NPs could have promising potential for biocompatible bactericidal and therapeutic applications. |
en_ZA |
dc.description.department |
Chemical Engineering |
en_ZA |
dc.description.librarian |
am2021 |
en_ZA |
dc.description.sponsorship |
The Claude Leon Foundation Postdoctoral Fellowship Program and the National Research Foundation (NRF) of South Africa through the Incentive Funding for Rated Researchers. |
en_ZA |
dc.description.uri |
http://www.nature.com/srep |
en_ZA |
dc.identifier.citation |
Bezza, F.A., Tichapondwa, S.M. & Chirwa, E.M.N. 2020, 'Fabrication of monodispersed copper oxide nanoparticles with potential application as antimicrobial agents', Scientific Reports, vol. 10, art. 16680, pp. 1-18. |
en_ZA |
dc.identifier.issn |
2045-2322 (online) |
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dc.identifier.other |
10.1038/s41598-020-73497-z |
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dc.identifier.uri |
http://hdl.handle.net/2263/81038 |
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dc.language.iso |
en |
en_ZA |
dc.publisher |
Nature Publishing Group |
en_ZA |
dc.rights |
© The Author(s) 2020.
This article is licensed under a Creative Commons Attribution 4.0 International
License. |
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dc.subject |
Minimal inhibitory concentration |
en_ZA |
dc.subject |
Lipopeptide |
en_ZA |
dc.subject |
Cuprous oxide nanoparticles (Cu2O NPs) |
en_ZA |
dc.subject |
Scanning electron microscopy (SEM) |
en_ZA |
dc.subject |
Transmission electron microscopy (TEM) |
en_ZA |
dc.subject |
Energy dispersive x-ray spectrum (EDX) |
en_ZA |
dc.title |
Fabrication of monodispersed copper oxide nanoparticles with potential application as antimicrobial agents |
en_ZA |
dc.type |
Article |
en_ZA |