Additional File 1: Complete metaphase spread of Cryptomys hottentotus mahali (male 12.3) chromosomes.
DNA is stained blue (with DAPI) and telomeres in red (with Cy3 PNA probe). 2n=53, as reported for males in other Cryptomys species (2n=54 for females due to a Y-chromosome-autosome translocation in males; Deuve et al., 2007). DOI: 10.7717/peerj.10498/supp-1
Additional File 2: Telomere size variation with age in naked mole-rats.
Scatter plot of mean ± SEM telomere size variation (expressed as volume of telomere fluorescence) with age for the naked mole-rats show in Figure 2. Points correspond to an individual animal (n=50 cells counted in each). DOI: 10.7717/peerj.10498/supp-2
Additional File 3: qFISH telomere fluorescence measurements.
Each value is the fluorescence measurement per cell together with individual identity and age class for each species (separate tabs) as follows: Damaraland mole-rat, DMR; Mahali mole-rat, MMR; and naked mole-rat, NMR.
DOI: 10.7717/peerj.10498/supp-3
Additional File 4: Details of Damaraland mole-rat Fukomys damarensis (DMR), Mahali mole-rat Cryptomy hottentotus mahali (MMR) and Heterocephalus glaber (NMR) samples.
Age class based on molar tooth eruption and wear for DMR and MMR (Bennett, Jarvis & Wallace, 1990), or absolute age in years for NMR. Age category as described in methods. Shaded rows correspond to NMR samples with too few cells to count, not included in the final analysis.
DOI: 10.7717/peerj.10498/supp-4