Comparative evaluation of CHROMagar COL-APSE, MicroScan walkaway, ComASP colistin, and Colistin MAC test in detecting colistin-resistant gram-negative bacteria

Show simple item record Osei Sekyere, John Sephofane, Arnold Karabo Mbelle, Nontombi Marylucy 2021-04-12T08:45:09Z 2021-04-12T08:45:09Z 2020-04-10
dc.description.abstract Colistin has become a critical antibiotic for fatal Gram-negative infections owing to the proliferation of multidrug-resistant carbapenemase-producing bacteria. Thus, cheaper, faster, efficient and easier-touse colistin diagnostics are required for clinical surveillance, diagnoses and therapeutics. The sensitivity, specificity, major error (ME), very major error (VME), categorial agreement, essential agreement, turnaround time (TAT), average cost, and required skill for four colistin resistance diagnostics viz., CHROMagar COL-APSE, ComASP Colistin, MicroScan, and Colistin MAC Test (CMT) were evaluated against broth microdilution (BMD) using 84 Gram-negative bacterial isolates. A multiplex PCR (M-PCR) was used to screen all isolates to detect the presence of the mcr-1 to mcr-5 genes. A 15-point grading scale was used to grade the tests under skill, ease, processing time etc. mcr-1 was detected by both M-PCR and CMT in a single E. coli isolate, with other PCR amplicons suggestive of mcr-2, -3 and -4 genes being also observed on the gel. The sensitivity and specificity of CHROMagar COL-APSE, MicroScan, and ComASP Colistin, were 82.05% and 66.67%, 92.31% and 76.92%, and 100% and 88.89% respectively. The MicroScan was the most expensive at a cost (per sampe tested) of R221.6 ($15.0), followed by CHROMagar COL-APSE (R118.3; $8.0), M-PCR (R75.1; $5.1), CMT (R20.1; $1.4) and ComASP Colistin (R2.64; $0.2). CHROMagar was the easiest to perform, followed by ComASP Colistin, M-PCR, MicroScan, CMT and BMD whilst M-PCR and MicroScan required higher skill. The ComASP Colistin was the best performing diagnostic test, with low VME and ME, making it recommendable for routine colistin sensitivity testing in clinical laboratories; particularly, in poorer settings. It is however limited by a TAT of 18–24 hours. en_ZA
dc.description.department Medical Microbiology en_ZA
dc.description.librarian am2021 en_ZA
dc.description.sponsorship The University of Pretoria and the National Health Laboratory Services (NHLS). en_ZA
dc.description.uri en_ZA
dc.identifier.citation Osei Sekyere, J., Sephofane, A.K. & Mbelle, N.M. Comparative Evaluation of CHROMagar COL-APSE, MicroScan Walkaway, ComASP Colistin, and Colistin MAC Test in Detecting Colistin-resistant Gram-Negative Bacteria. Scientific Reports 10, 6221 (2020). en_ZA
dc.identifier.issn 2045-2322 (online)
dc.identifier.other 10.1038/s41598-020-63267-2
dc.language.iso en en_ZA
dc.publisher Nature Publishing Group en_ZA
dc.rights © The Author(s) 2020. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License. en_ZA
dc.subject Colistin en_ZA
dc.subject Gram-negative infections en_ZA
dc.subject Bacteria en_ZA
dc.subject MicroScan en_ZA
dc.subject Bacterial infection en_ZA
dc.subject Clinical microbiology en_ZA
dc.subject Multidrug-resistant (MDR) en_ZA
dc.subject Carbapenemase-producing bacteria en_ZA
dc.title Comparative evaluation of CHROMagar COL-APSE, MicroScan walkaway, ComASP colistin, and Colistin MAC test in detecting colistin-resistant gram-negative bacteria en_ZA
dc.type Article en_ZA

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