The effect of iodine-containing nano-micelles, FS-1, on antibiotic resistance, gene expression and epigenetic modifications in the genome of multidrug resistant MRSA strain staphylococcus aureus ATCC BAA-39

Show simple item record Reva, Oleg N. Korotetskiy, Ilya S. Joubert, Monique Shilov, Sergey V. Jumagaziyeva, Ardak B. Suldina, Natalya A. Ilin, Alexandr I. 2021-04-12T07:09:18Z 2021-04-12T07:09:18Z 2020-10
dc.description.abstract Application of supplementary drugs which increase susceptibility of pathogenic bacteria to antibiotics is a promising yet unexplored approach to overcome the global problem of multidrug-resistant infections. The discovery of a new drug, an iodinecontaining nano-molecular complex FS-1, which has proven to improve susceptibility to antibiotics in various pathogens, including MRSA strain Staphylococcus aureus ATCC BAA-39TM, allowed studying this phenomenon. Chromosomal DNA and total RNA samples extracted from the FS-1 treated strain (FS) and from the negative control (NC) cultures were sequenced by PacBio SMRT and Ion Torrent technologies, respectively. PacBio DNA reads were used to assemble chromosomal DNA of the NC and FS variants of S. aureus BAA-39 and to perform profiling of epigenetically modified nucleotides. Results of transcriptional profiling, variant calling and detection of epigenetic modifications in the FS variant were compared to the NC variant. Additionally, the genetic alterations caused by the treatment of S. aureus BAA-39 with FS-1 were compared to the results of a similar experiment conducted with another model organism, E. coli ATCC BAA-196. Several commonalities in responses of these phylogenetically distant microorganisms to the treatment with FS-1 were discovered, which included metabolic transition toward anaerobiosis and oxidative/osmotic stress response. S. aureus culture appeared to be more sensitive to FS-1 due to a higher penetrability of cells by iodine bound compounds, which caused carbonyl stress associated with nucleotide damaging by FS-1, abnormal epigenetic modifications and an increased rate of mutations. It was hypothesized that the disrupted pattern of adenine methylated loci within methicillin-resistance chromosome cassettes (SCCmec) may promote excision of this antibiotic resistance determinant from chromosomes while the altered pattern of cytosine methylation was behind the adaptive gene regulation in the culture FS. The selection against the antibiotic resistance in bacterial populations caused by abnormal epigenetic modifications exemplifies possible mechanisms of antibiotic resistance reversion induced by iodine-containing compounds. These finding will facilitate development of therapeutic agents against multidrug-resistant infections. en_ZA
dc.description.department Biochemistry en_ZA
dc.description.department Genetics en_ZA
dc.description.department Microbiology and Plant Pathology en_ZA
dc.description.librarian pm2021 en_ZA
dc.description.sponsorship The South African National Research Foundation (NRF) and the Industrial development and industrial safety committee of the Ministry of Industry and Infrastructural Development of the Republic of Kazakhstan. en_ZA
dc.description.uri en_ZA
dc.identifier.citation Reva ON, Korotetskiy IS, Joubert M, Shilov SV, Jumagaziyeva AB, Suldina NA and Ilin AI (2020) The Effect of Iodine-Containing Nano-Micelles, FS-1, on Antibiotic Resistance, Gene Expression and Epigenetic Modifications in the Genome of Multidrug Resistant MRSA Strain Staphylococcus aureus ATCC BAA-39. Frontiers in Microbiology 11:581660. doi: 10.3389/fmicb.2020.581660 en_ZA
dc.identifier.issn 1664-302X (online)
dc.identifier.other 10.3389/fmicb.2020.581660
dc.language.iso en en_ZA
dc.publisher Frontiers Media en_ZA
dc.rights © 2020 Reva, Korotetskiy, Joubert, Shilov, Jumagaziyeva, Suldina and Ilin. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). en_ZA
dc.subject Staphylococcus aureus en_ZA
dc.subject Transcriptomics en_ZA
dc.subject Genomics en_ZA
dc.subject Epigenetics en_ZA
dc.subject Antibiotic resistance reversion en_ZA
dc.subject Iodine en_ZA
dc.subject Methicillin-resistant Staphylococcus aureus (MRSA) en_ZA
dc.subject FS-1 treated strain en_ZA
dc.title The effect of iodine-containing nano-micelles, FS-1, on antibiotic resistance, gene expression and epigenetic modifications in the genome of multidrug resistant MRSA strain staphylococcus aureus ATCC BAA-39 en_ZA
dc.type Article en_ZA

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