The effect of iodine-containing nano-micelles, FS-1, on antibiotic resistance, gene expression and epigenetic modifications in the genome of multidrug resistant MRSA strain staphylococcus aureus ATCC BAA-39

dc.contributor.authorReva, Oleg N.
dc.contributor.authorKorotetskiy, Ilya S.
dc.contributor.authorJoubert, Monique
dc.contributor.authorShilov, Sergey V.
dc.contributor.authorJumagaziyeva, Ardak B.
dc.contributor.authorSuldina, Natalya A.
dc.contributor.authorIlin, Alexandr I.
dc.contributor.emailoleg.reva@up.ac.zaen_ZA
dc.date.accessioned2021-04-12T07:09:18Z
dc.date.available2021-04-12T07:09:18Z
dc.date.issued2020-10
dc.description.abstractApplication of supplementary drugs which increase susceptibility of pathogenic bacteria to antibiotics is a promising yet unexplored approach to overcome the global problem of multidrug-resistant infections. The discovery of a new drug, an iodinecontaining nano-molecular complex FS-1, which has proven to improve susceptibility to antibiotics in various pathogens, including MRSA strain Staphylococcus aureus ATCC BAA-39TM, allowed studying this phenomenon. Chromosomal DNA and total RNA samples extracted from the FS-1 treated strain (FS) and from the negative control (NC) cultures were sequenced by PacBio SMRT and Ion Torrent technologies, respectively. PacBio DNA reads were used to assemble chromosomal DNA of the NC and FS variants of S. aureus BAA-39 and to perform profiling of epigenetically modified nucleotides. Results of transcriptional profiling, variant calling and detection of epigenetic modifications in the FS variant were compared to the NC variant. Additionally, the genetic alterations caused by the treatment of S. aureus BAA-39 with FS-1 were compared to the results of a similar experiment conducted with another model organism, E. coli ATCC BAA-196. Several commonalities in responses of these phylogenetically distant microorganisms to the treatment with FS-1 were discovered, which included metabolic transition toward anaerobiosis and oxidative/osmotic stress response. S. aureus culture appeared to be more sensitive to FS-1 due to a higher penetrability of cells by iodine bound compounds, which caused carbonyl stress associated with nucleotide damaging by FS-1, abnormal epigenetic modifications and an increased rate of mutations. It was hypothesized that the disrupted pattern of adenine methylated loci within methicillin-resistance chromosome cassettes (SCCmec) may promote excision of this antibiotic resistance determinant from chromosomes while the altered pattern of cytosine methylation was behind the adaptive gene regulation in the culture FS. The selection against the antibiotic resistance in bacterial populations caused by abnormal epigenetic modifications exemplifies possible mechanisms of antibiotic resistance reversion induced by iodine-containing compounds. These finding will facilitate development of therapeutic agents against multidrug-resistant infections.en_ZA
dc.description.departmentBiochemistryen_ZA
dc.description.departmentGeneticsen_ZA
dc.description.departmentMicrobiology and Plant Pathologyen_ZA
dc.description.librarianpm2021en_ZA
dc.description.sponsorshipThe South African National Research Foundation (NRF) and the Industrial development and industrial safety committee of the Ministry of Industry and Infrastructural Development of the Republic of Kazakhstan.en_ZA
dc.description.urihttp://frontiersin.org/Microbiologyen_ZA
dc.identifier.citationReva ON, Korotetskiy IS, Joubert M, Shilov SV, Jumagaziyeva AB, Suldina NA and Ilin AI (2020) The Effect of Iodine-Containing Nano-Micelles, FS-1, on Antibiotic Resistance, Gene Expression and Epigenetic Modifications in the Genome of Multidrug Resistant MRSA Strain Staphylococcus aureus ATCC BAA-39. Frontiers in Microbiology 11:581660. doi: 10.3389/fmicb.2020.581660en_ZA
dc.identifier.issn1664-302X (online)
dc.identifier.other10.3389/fmicb.2020.581660
dc.identifier.urihttp://hdl.handle.net/2263/79382
dc.language.isoenen_ZA
dc.publisherFrontiers Mediaen_ZA
dc.rights© 2020 Reva, Korotetskiy, Joubert, Shilov, Jumagaziyeva, Suldina and Ilin. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).en_ZA
dc.subjectStaphylococcus aureusen_ZA
dc.subjectTranscriptomicsen_ZA
dc.subjectGenomicsen_ZA
dc.subjectEpigeneticsen_ZA
dc.subjectAntibiotic resistance reversionen_ZA
dc.subjectIodineen_ZA
dc.subjectMethicillin-resistant Staphylococcus aureus (MRSA)en_ZA
dc.subjectFS-1 treated strainen_ZA
dc.titleThe effect of iodine-containing nano-micelles, FS-1, on antibiotic resistance, gene expression and epigenetic modifications in the genome of multidrug resistant MRSA strain staphylococcus aureus ATCC BAA-39en_ZA
dc.typeArticleen_ZA

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Riva_Effect_2020.pdf
Size:
11.22 MB
Format:
Adobe Portable Document Format
Description:
Article

License bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
license.txt
Size:
1.75 KB
Format:
Item-specific license agreed upon to submission
Description: