A rapid and simple single-step method for the purification of Toxoplasma gondii tachyzoites and bradyzoites

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dc.contributor.author El-Ashram, Saeed
dc.contributor.author Zhang, Yu
dc.contributor.author Ji, Yongsheng
dc.contributor.author Salama, Dina
dc.contributor.author Mei, Kun
dc.contributor.author Zhili, Li
dc.contributor.author Shujian, Huang
dc.contributor.author Zhang, Haoji
dc.contributor.author Aboelhadid, Shawky M.
dc.contributor.author Alajmi, Reem A.
dc.contributor.author Metwally, Dina M.
dc.contributor.author El-Khadragy, Manal F.
dc.contributor.author Hargis, Billy M.
dc.contributor.author Tellez-Isaias, Guillermo
dc.contributor.author Cenci-Goga, Beniamino T.
dc.contributor.author Karama, Musafiri
dc.contributor.author Marufu, Munyaradzi C.
dc.contributor.author Abouhajer, Fathi
dc.contributor.author Hamady, Gamal Ali Abdelhafez
dc.contributor.author El Wakil, Abeer
dc.contributor.author Al Nasr, Ibrahim
dc.contributor.author Suo, Xun
dc.date.accessioned 2021-02-15T08:23:47Z
dc.date.available 2021-02-15T08:23:47Z
dc.date.issued 2020
dc.description.abstract This study describes a simple method for the large-scale isolation of pure Toxoplasma gondii tachyzoites and bradyzoites. T. gondii tachyzoites were obtained from infected human foreskin fibroblasts (HFFs) and peritoneal exudates of mice, while tissue cysts containing bradyzoites were collected from chronically infected mice. Harvested cells and brain tissues were incubated in Hanks balanced salt solution (HBSS), containing 0.25% trypsin and 0.5% taurodeoxycholic acid (TDC) for 5 min. Subsequent washes in phosphate buffered saline (PBS) were conducted, and the cell viability of the preparations was good, as determined by flow cytometry and ability to reinfect HFF cells and propagate in mice. The purification procedure allowed for a rapid preparation of pure T. gondii tachyzoites and bradyzoites in sufficient quantity that can be used for downstream procedures. The advantage of the new method is that it is convenient and inexpensive. en_ZA
dc.description.department Paraclinical Sciences en_ZA
dc.description.librarian am2021 en_ZA
dc.description.sponsorship The National Natural Science Foundation of China (No. 31502071), Youth Innovative Talents Project of Guangdong province Education Department (No. 2017KQNCX212), Guangdong province (2017GDK07), Start-up Research Grant Program provided by Foshan University, Foshan city, Guangdong province for distinguished researchers, Guangdong Science and Technology Plan Project (Grant No: 1244060045607389XC), and School of Life Science and Engineering fund (Grant No: KLPREAD201801-02). en_ZA
dc.description.uri http://www.wileyonlinelibrary.com/journal/vms3 en_ZA
dc.identifier.citation El-Ashram S, Zhang Y, Ji Y, et al. A rapid and simple single-step method for the purification of Toxoplasma gondii tachyzoites and bradyzoites. Veterinary Medicine and Science 2020;00:1–5. https://DOI.org/10.1002/vms3.364. NYP. en_ZA
dc.identifier.issn 2053-1095 (online)
dc.identifier.other 10.1002/vms3.364
dc.identifier.uri http://hdl.handle.net/2263/78554
dc.language.iso en en_ZA
dc.publisher Wiley Open Access en_ZA
dc.rights © 2020 The Authors. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License. en_ZA
dc.subject Flow cytometry en_ZA
dc.subject Purification en_ZA
dc.subject T. gondii tachyzoites and bradyzoites en_ZA
dc.subject Trypsin en_ZA
dc.subject Taurodeoxycholic acid en_ZA
dc.subject Taurodeoxycholic acid (TDC) en_ZA
dc.title A rapid and simple single-step method for the purification of Toxoplasma gondii tachyzoites and bradyzoites en_ZA
dc.type Article en_ZA


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