A rapid and simple single-step method for the purification of Toxoplasma gondii tachyzoites and bradyzoites

dc.contributor.authorEl-Ashram, Saeed
dc.contributor.authorZhang, Yu
dc.contributor.authorJi, Yongsheng
dc.contributor.authorSalama, Dina
dc.contributor.authorMei, Kun
dc.contributor.authorZhili, Li
dc.contributor.authorShujian, Huang
dc.contributor.authorZhang, Haoji
dc.contributor.authorAboelhadid, Shawky M.
dc.contributor.authorAlajmi, Reem A.
dc.contributor.authorMetwally, Dina M.
dc.contributor.authorEl-Khadragy, Manal F.
dc.contributor.authorHargis, Billy M.
dc.contributor.authorTellez-Isaias, Guillermo
dc.contributor.authorCenci-Goga, Beniamino T.
dc.contributor.authorKarama, Musafiri
dc.contributor.authorMarufu, Munyaradzi Christopher
dc.contributor.authorAbouhajer, Fathi
dc.contributor.authorHamady, Gamal Ali Abdelhafez
dc.contributor.authorEl Wakil, Abeer
dc.contributor.authorAl Nasr, Ibrahim
dc.contributor.authorSuo, Xun
dc.date.accessioned2021-02-15T08:23:47Z
dc.date.available2021-02-15T08:23:47Z
dc.date.issued2021-03
dc.description.abstractThis study describes a simple method for the large-scale isolation of pure Toxoplasma gondii tachyzoites and bradyzoites. T. gondii tachyzoites were obtained from infected human foreskin fibroblasts (HFFs) and peritoneal exudates of mice, while tissue cysts containing bradyzoites were collected from chronically infected mice. Harvested cells and brain tissues were incubated in Hanks balanced salt solution (HBSS), containing 0.25% trypsin and 0.5% taurodeoxycholic acid (TDC) for 5 min. Subsequent washes in phosphate buffered saline (PBS) were conducted, and the cell viability of the preparations was good, as determined by flow cytometry and ability to reinfect HFF cells and propagate in mice. The purification procedure allowed for a rapid preparation of pure T. gondii tachyzoites and bradyzoites in sufficient quantity that can be used for downstream procedures. The advantage of the new method is that it is convenient and inexpensive.en_ZA
dc.description.departmentParaclinical Sciencesen_ZA
dc.description.librarianam2021en_ZA
dc.description.sponsorshipThe National Natural Science Foundation of China (No. 31502071), Youth Innovative Talents Project of Guangdong province Education Department (No. 2017KQNCX212), Guangdong province (2017GDK07), Start-up Research Grant Program provided by Foshan University, Foshan city, Guangdong province for distinguished researchers, Guangdong Science and Technology Plan Project (Grant No: 1244060045607389XC), and School of Life Science and Engineering fund (Grant No: KLPREAD201801-02).en_ZA
dc.description.urihttp://www.wileyonlinelibrary.com/journal/vms3en_ZA
dc.identifier.citationEl-Ashram S, Zhang Y, Ji Y, et al. A rapid and simple single-step method for the purification of Toxoplasma gondii tachyzoites and bradyzoites. Veterinary Medicine and Science 2021;7(2):357-361. https://DOI.org/10.1002/vms3.364.en_ZA
dc.identifier.issn2053-1095 (online)
dc.identifier.other10.1002/vms3.364
dc.identifier.urihttp://hdl.handle.net/2263/78554
dc.language.isoenen_ZA
dc.publisherWiley Open Accessen_ZA
dc.rights© 2020 The Authors. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License.en_ZA
dc.subjectFlow cytometryen_ZA
dc.subjectPurificationen_ZA
dc.subjectT. gondii tachyzoites and bradyzoitesen_ZA
dc.subjectTrypsinen_ZA
dc.subjectTaurodeoxycholic aciden_ZA
dc.subjectTaurodeoxycholic acid (TDC)en_ZA
dc.titleA rapid and simple single-step method for the purification of Toxoplasma gondii tachyzoites and bradyzoitesen_ZA
dc.typeArticleen_ZA

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