Molecular characterization Brucella species from Zimbabwe

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dc.contributor.author Ledwaba, Maphuti Betty
dc.contributor.author Gomo, Calvin
dc.contributor.author Lekota, Kgaugelo Edward
dc.contributor.author Le Flèche, Philippe
dc.contributor.author Hassim, Ayesha
dc.contributor.author Vergnaud, Gilles
dc.contributor.author Van Heerden, Henriette
dc.date.accessioned 2020-07-11T07:49:41Z
dc.date.available 2020-07-11T07:49:41Z
dc.date.issued 2019-05-20
dc.description S1 Table. Reference strains and Zimbabwean Brucella spp. isolates identified by Bruce-ladder and repeat copy number of the indicated loci. https://doi.org/10.1371/journal.pntd.0007311.s001 en_ZA
dc.description S2 Table. Brucella abortus and B. suis genome sequences retrieved from GenBank, used in the study for comparison of whole genome single nucleotide polymorphisms (WGS-SNPS) phylogenetic analysis. https://doi.org/10.1371/journal.pntd.0007311.s002 en_ZA
dc.description S1 Fig. Suis-ladder multiplex PCR assay of Brucella DNA from Zimbabwe and reference strains. https://doi.org/10.1371/journal.pntd.0007311.s003 en_ZA
dc.description S2 Fig. Minimum spanning tree analysis of published data and Zimbabwean Brucella isolates using the MLVA8 data (Panel 1 genotypes). https://doi.org/10.1371/journal.pntd.0007311.s004 en_ZA
dc.description.abstract Brucella abortus and B. melitensis have been reported in several studies in animals in Zimbabwe but the extent of the disease remains poorly known. Thus, characterizing the circulating strains is a critical first step in understanding brucellosis in the country. In this study we used an array of molecular assays including AMOS-PCR, Bruce-ladder, multiple locus variable number tandem repeats analysis (MLVA) and single nucleotide polymorphisms from whole genome sequencing (WGS-SNP) to characterize Brucella isolates to the species, biovar, and individual strain level. Sixteen Brucella strains isolated in Zimbabwe at the Central Veterinary laboratory from various hosts were characterized using all or some of these assays. The strains were identified as B. ovis, B. abortus, B. canis and B. suis, with B. canis being the first report of this species in Zimbabwe. Zimbabwean strains identified as B. suis and B. abortus were further characterized with whole genome sequencing and were closely related to reference strains 1330 and 86/8/59, respectively. We demonstrate the range of different tests that can be performed from simple assays that can be run in laboratories lacking sophisticated instrumentation to whole genome analyses that currently require substantial expertise and infrastructure often not available in the developing world. en_ZA
dc.description.department Veterinary Tropical Diseases en_ZA
dc.description.librarian hj2020 en_ZA
dc.description.sponsorship The ITM Belgium and National Research Foundation (NRF South Africa). Work by PLF and GV is supported by the ANR project Microtype-14-ASMA-0002-02. en_ZA
dc.description.uri http://www.plosntds.org en_ZA
dc.identifier.citation Ledwaba, M.B., Gomo, C., Lekota, K.E. et al. Molecular characterization of Brucella species from Zimbabwe. PLoS Neglected Tropical Diseases, 2019, 13(5): e0007311. https://DOI.org/10.1371/journal.pntd.0007311. en_ZA
dc.identifier.issn 1935-2727 (print)
dc.identifier.issn 1935-2735 (online)
dc.identifier.other 10.1371/journal.pntd.0007311
dc.identifier.uri http://hdl.handle.net/2263/75147
dc.language.iso en en_ZA
dc.publisher Public Library of Science en_ZA
dc.rights © 2019 Ledwaba et al. This is an open access article distributed under the terms of the Creative Commons Attribution License. en_ZA
dc.subject Molecular characterization en_ZA
dc.subject Brucella en_ZA
dc.subject Zimbabwe en_ZA
dc.subject AMOS-PCR en_ZA
dc.subject Bruce-ladder en_ZA
dc.subject Multiple locus variable number tandem repeats analysis (MLVA) en_ZA
dc.subject Single nucleotide polymorphisms en_ZA
dc.subject Whole genome sequencing (WGS-SNP) en_ZA
dc.title Molecular characterization Brucella species from Zimbabwe en_ZA
dc.type Article en_ZA


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