Molecular characterization Brucella species from Zimbabwe

dc.contributor.authorLedwaba, Maphuti Betty
dc.contributor.authorGomo, Calvin
dc.contributor.authorLekota, Kgaugelo Edward
dc.contributor.authorLe Flèche, Philippe
dc.contributor.authorHassim, Ayesha
dc.contributor.authorVergnaud, Gilles
dc.contributor.authorVan Heerden, Henriette
dc.contributor.emailhenriette.vanheerden@up.ac.zaen_ZA
dc.date.accessioned2020-07-11T07:49:41Z
dc.date.available2020-07-11T07:49:41Z
dc.date.issued2019-05-20
dc.descriptionS1 Table. Reference strains and Zimbabwean Brucella spp. isolates identified by Bruce-ladder and repeat copy number of the indicated loci. https://doi.org/10.1371/journal.pntd.0007311.s001en_ZA
dc.descriptionS2 Table. Brucella abortus and B. suis genome sequences retrieved from GenBank, used in the study for comparison of whole genome single nucleotide polymorphisms (WGS-SNPS) phylogenetic analysis. https://doi.org/10.1371/journal.pntd.0007311.s002en_ZA
dc.descriptionS1 Fig. Suis-ladder multiplex PCR assay of Brucella DNA from Zimbabwe and reference strains. https://doi.org/10.1371/journal.pntd.0007311.s003en_ZA
dc.descriptionS2 Fig. Minimum spanning tree analysis of published data and Zimbabwean Brucella isolates using the MLVA8 data (Panel 1 genotypes). https://doi.org/10.1371/journal.pntd.0007311.s004en_ZA
dc.description.abstractBrucella abortus and B. melitensis have been reported in several studies in animals in Zimbabwe but the extent of the disease remains poorly known. Thus, characterizing the circulating strains is a critical first step in understanding brucellosis in the country. In this study we used an array of molecular assays including AMOS-PCR, Bruce-ladder, multiple locus variable number tandem repeats analysis (MLVA) and single nucleotide polymorphisms from whole genome sequencing (WGS-SNP) to characterize Brucella isolates to the species, biovar, and individual strain level. Sixteen Brucella strains isolated in Zimbabwe at the Central Veterinary laboratory from various hosts were characterized using all or some of these assays. The strains were identified as B. ovis, B. abortus, B. canis and B. suis, with B. canis being the first report of this species in Zimbabwe. Zimbabwean strains identified as B. suis and B. abortus were further characterized with whole genome sequencing and were closely related to reference strains 1330 and 86/8/59, respectively. We demonstrate the range of different tests that can be performed from simple assays that can be run in laboratories lacking sophisticated instrumentation to whole genome analyses that currently require substantial expertise and infrastructure often not available in the developing world.en_ZA
dc.description.departmentVeterinary Tropical Diseasesen_ZA
dc.description.librarianhj2020en_ZA
dc.description.sponsorshipThe ITM Belgium and National Research Foundation (NRF South Africa). Work by PLF and GV is supported by the ANR project Microtype-14-ASMA-0002-02.en_ZA
dc.description.urihttp://www.plosntds.orgen_ZA
dc.identifier.citationLedwaba, M.B., Gomo, C., Lekota, K.E. et al. Molecular characterization of Brucella species from Zimbabwe. PLoS Neglected Tropical Diseases, 2019, 13(5): e0007311. https://DOI.org/10.1371/journal.pntd.0007311.en_ZA
dc.identifier.issn1935-2727 (print)
dc.identifier.issn1935-2735 (online)
dc.identifier.other10.1371/journal.pntd.0007311
dc.identifier.urihttp://hdl.handle.net/2263/75147
dc.language.isoenen_ZA
dc.publisherPublic Library of Scienceen_ZA
dc.rights© 2019 Ledwaba et al. This is an open access article distributed under the terms of the Creative Commons Attribution License.en_ZA
dc.subjectMolecular characterizationen_ZA
dc.subjectBrucellaen_ZA
dc.subjectZimbabween_ZA
dc.subjectAMOS-PCRen_ZA
dc.subjectBruce-ladderen_ZA
dc.subjectMultiple locus variable number tandem repeats analysis (MLVA)en_ZA
dc.subjectSingle nucleotide polymorphismsen_ZA
dc.subjectWhole genome sequencing (WGS)en_ZA
dc.titleMolecular characterization Brucella species from Zimbabween_ZA
dc.typeArticleen_ZA

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