Antimicrobial activity, antioxidant potential, cytotoxicity and phytochemical profiling of four plants locally used against skin diseases

Show simple item record Asong, John A. Amoo, Stephen O. McGaw, Lyndy Joy Nkadimeng, Sanah Malomile Aremu, Adeyemi O. Otang-Mbeng, Wilfred 2020-07-09T13:52:40Z 2020-07-09T13:52:40Z 2019-09-15
dc.description.abstract Although orthodox medications are available for skin diseases, expensive dermatological services have necessitated the use of medicinal plants as a cheaper alternative. This study evaluated the pharmacological and phytochemical profiles of four medicinal plants (Drimia sanguinea, Elephantorrhiza elephantina, Helichrysum paronychioides, and Senecio longiflorus) used for treating skin diseases. Petroleum ether and 50% methanol extracts of the plants were screened for antimicrobial activity against six microbes: Bacillus cereus, Shigella flexneri, Candida glabrata, Candida krusei, Trichophyton rubrum and Trichophyton tonsurans using the micro-dilution technique. Antioxidant activity was conducted using 2,2-diphenyl-1-picryhydrazyl (DPPH) free radical scavenging and β-carotene linoleic acid models. Cytotoxicity was determined against African green monkey Vero kidney cells based on the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay. Spectrophotometric and Gas Chromatography-Mass Spectrometry (GC-MS) methods were used to evaluate the phytochemical constituents. All the extracts demonstrated varying degrees of antimicrobial potencies. Shigella flexneri, Candida glabrata, Trichophyton rubrum and Trichophyton tonsurans were most susceptible at 0.10 mg/mL. In the DPPH test, EC50 values ranged from approximately 6–93 µg/mL and 65%–85% antioxidant activity in the β-carotene linoleic acid antioxidant activity model. The phenolic and flavonoid contents ranged from 3.5–64 mg GAE/g and 1.25–28 mg CE/g DW, respectively. The LC50 values of the cytotoxicity assay ranged from 0.015–5622 µg/mL. GC-MS analysis revealed a rich pool (94–198) of bioactive compounds including dotriacontane, benzothiazole, heptacosane, bumetrizole, phthalic acid, stigmasterol, hexanoic acid and eicosanoic acid, which were common to the four plants. The current findings provide some degree of scientific evidence supporting the use of these four plants in folk medicine. However, the plants with high cytotoxicity need to be used with caution en_ZA
dc.description.department Paraclinical Sciences en_ZA
dc.description.librarian hj2020 en_ZA
dc.description.sponsorship National Research Foundation, Department of Science and Technology, North West University and University of Mpumalanga. en_ZA
dc.description.uri en_ZA
dc.identifier.citation Asong, J.A., Amoo, S.O., McGaw, L.J. et al. Antimicrobial Activity, Antioxidant Potential, Cytotoxicity and Phytochemical Profiling of Four Plants Locally Used against Skin Diseases. Plants 2019, 8(9), 350. en_ZA
dc.identifier.issn 2223-7747 (online)
dc.identifier.other 10.3390/plants8090350
dc.language.iso en en_ZA
dc.publisher MDPI en_ZA
dc.rights © 2019 by the Authors. Licensee: MDPI Basel, Switzerland. This is an open access article distributed under the Creative Commons Attribution (CC BY) license. en_ZA
dc.subject Antioxidant en_ZA
dc.subject Antibacterial en_ZA
dc.subject Antifungal en_ZA
dc.subject Flavonoids en_ZA
dc.subject Minimum inhibitory concentration en_ZA
dc.subject Phenols en_ZA
dc.subject Gas chromatography-mass spectrometry en_ZA
dc.title Antimicrobial activity, antioxidant potential, cytotoxicity and phytochemical profiling of four plants locally used against skin diseases en_ZA
dc.type Article en_ZA

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