Antimicrobial activity, antioxidant potential, cytotoxicity and phytochemical profiling of four plants locally used against skin diseases

dc.contributor.authorAsong, John A.
dc.contributor.authorAmoo, Stephen O.
dc.contributor.authorMcGaw, Lyndy Joy
dc.contributor.authorNkadimeng, Sanah Malomile
dc.contributor.authorAremu, Adeyemi O.
dc.contributor.authorOtang-Mbeng, Wilfred
dc.contributor.emaillyndy.mcgaw@up.ac.zaen_ZA
dc.date.accessioned2020-07-09T13:52:40Z
dc.date.available2020-07-09T13:52:40Z
dc.date.issued2019-09-15
dc.description.abstractAlthough orthodox medications are available for skin diseases, expensive dermatological services have necessitated the use of medicinal plants as a cheaper alternative. This study evaluated the pharmacological and phytochemical profiles of four medicinal plants (Drimia sanguinea, Elephantorrhiza elephantina, Helichrysum paronychioides, and Senecio longiflorus) used for treating skin diseases. Petroleum ether and 50% methanol extracts of the plants were screened for antimicrobial activity against six microbes: Bacillus cereus, Shigella flexneri, Candida glabrata, Candida krusei, Trichophyton rubrum and Trichophyton tonsurans using the micro-dilution technique. Antioxidant activity was conducted using 2,2-diphenyl-1-picryhydrazyl (DPPH) free radical scavenging and β-carotene linoleic acid models. Cytotoxicity was determined against African green monkey Vero kidney cells based on the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay. Spectrophotometric and Gas Chromatography-Mass Spectrometry (GC-MS) methods were used to evaluate the phytochemical constituents. All the extracts demonstrated varying degrees of antimicrobial potencies. Shigella flexneri, Candida glabrata, Trichophyton rubrum and Trichophyton tonsurans were most susceptible at 0.10 mg/mL. In the DPPH test, EC50 values ranged from approximately 6–93 µg/mL and 65%–85% antioxidant activity in the β-carotene linoleic acid antioxidant activity model. The phenolic and flavonoid contents ranged from 3.5–64 mg GAE/g and 1.25–28 mg CE/g DW, respectively. The LC50 values of the cytotoxicity assay ranged from 0.015–5622 µg/mL. GC-MS analysis revealed a rich pool (94–198) of bioactive compounds including dotriacontane, benzothiazole, heptacosane, bumetrizole, phthalic acid, stigmasterol, hexanoic acid and eicosanoic acid, which were common to the four plants. The current findings provide some degree of scientific evidence supporting the use of these four plants in folk medicine. However, the plants with high cytotoxicity need to be used with cautionen_ZA
dc.description.departmentParaclinical Sciencesen_ZA
dc.description.librarianhj2020en_ZA
dc.description.sponsorshipNational Research Foundation, Department of Science and Technology, North West University and University of Mpumalanga.en_ZA
dc.description.urihttps://www.mdpi.com/journal/plantsen_ZA
dc.identifier.citationAsong, J.A., Amoo, S.O., McGaw, L.J. et al. Antimicrobial Activity, Antioxidant Potential, Cytotoxicity and Phytochemical Profiling of Four Plants Locally Used against Skin Diseases. Plants 2019, 8(9), 350. https://DOI.org/10.3390/plants8090350.en_ZA
dc.identifier.issn2223-7747 (online)
dc.identifier.other10.3390/plants8090350
dc.identifier.urihttp://hdl.handle.net/2263/75117
dc.language.isoenen_ZA
dc.publisherMDPIen_ZA
dc.rights© 2019 by the Authors. Licensee: MDPI Basel, Switzerland. This is an open access article distributed under the Creative Commons Attribution (CC BY) license.en_ZA
dc.subjectAntioxidanten_ZA
dc.subjectAntibacterialen_ZA
dc.subjectAntifungalen_ZA
dc.subjectFlavonoidsen_ZA
dc.subjectMinimum inhibitory concentrationen_ZA
dc.subjectPhenolsen_ZA
dc.subjectGas chromatography-mass spectrometry (GC-MS)en_ZA
dc.titleAntimicrobial activity, antioxidant potential, cytotoxicity and phytochemical profiling of four plants locally used against skin diseasesen_ZA
dc.typeArticleen_ZA

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Asong_Antimicrobial_2019.pdf
Size:
524.45 KB
Format:
Adobe Portable Document Format
Description:
Article

License bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
license.txt
Size:
1.75 KB
Format:
Item-specific license agreed upon to submission
Description: