The effect of glucose and nitrogen supplementation on cell metabolic activity and the reduction of selenite to elemental selenium by Pseudomonas stutzeri NT-I

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dc.contributor.author Tendenedzai, Job Tatenda
dc.contributor.author Brink, Hendrik Gideon
dc.date.accessioned 2020-04-29T07:17:43Z
dc.date.available 2020-04-29T07:17:43Z
dc.date.issued 2019
dc.description.abstract Selenite (Se(IV)) readily bio-accumulates as compared to elemental selenium (Se(0)) which is considered to be biologically inert and relatively less toxic, therefore its microbial reduction from selenium laden waters is imperative. This study investigated the effect of glucose (carbon source), NH4Cl (nitrogen source) and the metabolic activity on the reduction of 2 mM Se(IV) by Pseudomonas stutzeri NT-I in order to establish the mechanism employed the bacterium. The experiments were performed aerobically under previously determined optimum conditions. Four batches were compared. These were A) glucose (10 g.L-1) and ammonium chloride (1.604 g.L−1), B) glucose only (10 g.L-1), C) no added glucose or nitrogen, and D) 1 M of the metabolic inhibitor, sodium azide (NaN3). The highest average biomass-based selenite reduction rate of 0.1061 mmol.(g.h) −1 was in the presence of glucose and nitrogen as compared to 0.0638 mmol.(g.h)−1 in the presence of glucose alone. The reduction rate decreased in the batches which did not have either of the nutrients. The lowest average biomass-based reduction rate of 0.026 mmol.(g.h)-1 was measured for the batch which had the NaN3. The overall Se(IV) removal followed a similar trend to the one observed with reduction rate, with highest reduction of 90.83 % recorded in the first batch and the lowest of 35.68 % being in the presence of NaN3. en_ZA
dc.description.department Chemical Engineering en_ZA
dc.description.librarian am2020 en_ZA
dc.description.uri http://www.aidic.it/cet en_ZA
dc.identifier.citation Tendenedzai J.T., Brink H.G., 2019, The Effect of Glucose and Nitrogen Supplementation on Cell Metabolic Activity and the Reduction of Selenite to Elemental Selenium by Pseudomonas Stutzeri NT-I, Chemical Engineering Transactions, 76, 1315-1320 DOI: 10.3303/CET1976220. en_ZA
dc.identifier.issn 2283-9216 (online)
dc.identifier.other 10.3303/CET1976220
dc.identifier.uri http://hdl.handle.net/2263/74418
dc.language.iso en en_ZA
dc.publisher The Italian Association of Chemical Engineering en_ZA
dc.rights © 2019, AIDIC Servizi S.r.l. en_ZA
dc.subject Cell metabolic en_ZA
dc.subject Glucose en_ZA
dc.subject Nitrogen en_ZA
dc.subject Pseudomonas stutzeri NT-I en_ZA
dc.title The effect of glucose and nitrogen supplementation on cell metabolic activity and the reduction of selenite to elemental selenium by Pseudomonas stutzeri NT-I en_ZA
dc.type Article en_ZA


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