The effect of glucose and nitrogen supplementation on cell metabolic activity and the reduction of selenite to elemental selenium by Pseudomonas stutzeri NT-I

dc.contributor.authorTendenedzai, Job Tatenda
dc.contributor.authorBrink, Hendrik Gideon
dc.contributor.emaildeon.brink@up.ac.zaen_ZA
dc.date.accessioned2020-04-29T07:17:43Z
dc.date.available2020-04-29T07:17:43Z
dc.date.issued2019
dc.description.abstractSelenite (Se(IV)) readily bio-accumulates as compared to elemental selenium (Se(0)) which is considered to be biologically inert and relatively less toxic, therefore its microbial reduction from selenium laden waters is imperative. This study investigated the effect of glucose (carbon source), NH4Cl (nitrogen source) and the metabolic activity on the reduction of 2 mM Se(IV) by Pseudomonas stutzeri NT-I in order to establish the mechanism employed the bacterium. The experiments were performed aerobically under previously determined optimum conditions. Four batches were compared. These were A) glucose (10 g.L-1) and ammonium chloride (1.604 g.L−1), B) glucose only (10 g.L-1), C) no added glucose or nitrogen, and D) 1 M of the metabolic inhibitor, sodium azide (NaN3). The highest average biomass-based selenite reduction rate of 0.1061 mmol.(g.h) −1 was in the presence of glucose and nitrogen as compared to 0.0638 mmol.(g.h)−1 in the presence of glucose alone. The reduction rate decreased in the batches which did not have either of the nutrients. The lowest average biomass-based reduction rate of 0.026 mmol.(g.h)-1 was measured for the batch which had the NaN3. The overall Se(IV) removal followed a similar trend to the one observed with reduction rate, with highest reduction of 90.83 % recorded in the first batch and the lowest of 35.68 % being in the presence of NaN3.en_ZA
dc.description.departmentChemical Engineeringen_ZA
dc.description.librarianam2020en_ZA
dc.description.urihttp://www.aidic.it/ceten_ZA
dc.identifier.citationTendenedzai J.T., Brink H.G., 2019, The Effect of Glucose and Nitrogen Supplementation on Cell Metabolic Activity and the Reduction of Selenite to Elemental Selenium by Pseudomonas Stutzeri NT-I, Chemical Engineering Transactions, 76, 1315-1320 DOI: 10.3303/CET1976220.en_ZA
dc.identifier.issn2283-9216 (online)
dc.identifier.other10.3303/CET1976220
dc.identifier.urihttp://hdl.handle.net/2263/74418
dc.language.isoenen_ZA
dc.publisherThe Italian Association of Chemical Engineeringen_ZA
dc.rights© 2019, AIDIC Servizi S.r.l.en_ZA
dc.subjectCell metabolicen_ZA
dc.subjectGlucoseen_ZA
dc.subjectNitrogenen_ZA
dc.subjectPseudomonas stutzeri NT-Ien_ZA
dc.titleThe effect of glucose and nitrogen supplementation on cell metabolic activity and the reduction of selenite to elemental selenium by Pseudomonas stutzeri NT-Ien_ZA
dc.typeArticleen_ZA

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