Mutations in glucan, water dikinase affect starch degradation and gametophore development in the moss Physcomitrella patens

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dc.contributor.author Mdodana, Ntombizanele T.
dc.contributor.author Jewell, Jonathan F.
dc.contributor.author Phiri, Ethel E.
dc.contributor.author Smith, Marthinus L.
dc.contributor.author Oberlander, K.C. (Kenneth)
dc.contributor.author Mahmoodi, S.
dc.contributor.author Kossmann, J.
dc.contributor.author Lloyd, J.R.
dc.date.accessioned 2020-01-29T08:48:30Z
dc.date.available 2020-01-29T08:48:30Z
dc.date.issued 2019-12
dc.description.abstract The role of starch degradation in non-vascular plants is poorly understood. To expand our knowledge of this area, we have studied this process in Physcomitrella patens. This has been achieved through examination of the step known to initiate starch degradation in angiosperms, glucan phosphorylation, catalysed by glucan, water dikinase (GWD) enzymes. Phylogenetic analysis indicates that GWD isoforms can be divided into two clades, one of which contains GWD1/GWD2 and the other GWD3 isoforms. These clades split at a very early stage within plant evolution, as distinct sequences that cluster within each were identified in all major plant lineages. Of the five genes we identified within the Physcomitrella genome that encode GWD-like enzymes, two group within the GWD1/GWD2 clade and the others within the GWD3 clade. Proteins encoded by both loci in the GWD1/GWD2 clade, named PpGWDa and PpGWDb, are localised in plastids. Mutations of either PpGWDa or PpGWDb reduce starch phosphate abundance, however, a mutation at the PpGWDa locus had a much greater influence than one at PpGWDb. Only mutations affecting PpGWDa inhibited starch degradation. Mutants lacking this enzyme also failed to develop gametophores, a phenotype that could be chemically complemented using glucose supplementation within the growth medium. en_ZA
dc.description.department Plant Production and Soil Science en_ZA
dc.description.librarian am2020 en_ZA
dc.description.sponsorship The Swiss/South African Joint Research Program grant 87391 and the NRF SARCHI chair “Genetic Tailoring of Biopolymers”. Bursary funding was obtained from the South African National Research Foundation for Ms Mdodana (SFH13091440165) and Mr Jewell (SFH14072881440). en_ZA
dc.description.uri http://www.nature.com/srep en_ZA
dc.identifier.citation Mdodana, N.T., Jewell, J.F., Phiri, E.E. et al. 2019, 'Mutations in glucan, water dikinase affect starch degradation and gametophore development in the moss Physcomitrella patens', Scientific Reports, vol. 9, art. 15114, pp. 1-13. en_ZA
dc.identifier.issn 2045-2322 (online)
dc.identifier.other 10.1038/s41598-019-51632-9
dc.identifier.uri http://hdl.handle.net/2263/73009
dc.language.iso en en_ZA
dc.publisher Nature Publishing Group en_ZA
dc.rights © The Author(s) 2019. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License. en_ZA
dc.subject Starch degradation en_ZA
dc.subject Plants en_ZA
dc.subject Physcomitrella patens en_ZA
dc.subject Glucan, water dikinase (GWD) en_ZA
dc.title Mutations in glucan, water dikinase affect starch degradation and gametophore development in the moss Physcomitrella patens en_ZA
dc.type Article en_ZA


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