Mutations in glucan, water dikinase affect starch degradation and gametophore development in the moss Physcomitrella patens

dc.contributor.authorMdodana, Ntombizanele T.
dc.contributor.authorJewell, Jonathan F.
dc.contributor.authorPhiri, Ethel E.
dc.contributor.authorSmith, Marthinus L.
dc.contributor.authorOberlander, Kenneth Carl
dc.contributor.authorMahmoodi, S.
dc.contributor.authorKossmann, J.
dc.contributor.authorLloyd, J.R.
dc.date.accessioned2020-01-29T08:48:30Z
dc.date.available2020-01-29T08:48:30Z
dc.date.issued2019-12
dc.description.abstractThe role of starch degradation in non-vascular plants is poorly understood. To expand our knowledge of this area, we have studied this process in Physcomitrella patens. This has been achieved through examination of the step known to initiate starch degradation in angiosperms, glucan phosphorylation, catalysed by glucan, water dikinase (GWD) enzymes. Phylogenetic analysis indicates that GWD isoforms can be divided into two clades, one of which contains GWD1/GWD2 and the other GWD3 isoforms. These clades split at a very early stage within plant evolution, as distinct sequences that cluster within each were identified in all major plant lineages. Of the five genes we identified within the Physcomitrella genome that encode GWD-like enzymes, two group within the GWD1/GWD2 clade and the others within the GWD3 clade. Proteins encoded by both loci in the GWD1/GWD2 clade, named PpGWDa and PpGWDb, are localised in plastids. Mutations of either PpGWDa or PpGWDb reduce starch phosphate abundance, however, a mutation at the PpGWDa locus had a much greater influence than one at PpGWDb. Only mutations affecting PpGWDa inhibited starch degradation. Mutants lacking this enzyme also failed to develop gametophores, a phenotype that could be chemically complemented using glucose supplementation within the growth medium.en_ZA
dc.description.departmentPlant Production and Soil Scienceen_ZA
dc.description.librarianam2020en_ZA
dc.description.sponsorshipThe Swiss/South African Joint Research Program grant 87391 and the NRF SARCHI chair “Genetic Tailoring of Biopolymers”. Bursary funding was obtained from the South African National Research Foundation for Ms Mdodana (SFH13091440165) and Mr Jewell (SFH14072881440).en_ZA
dc.description.urihttp://www.nature.com/srepen_ZA
dc.identifier.citationMdodana, N.T., Jewell, J.F., Phiri, E.E. et al. 2019, 'Mutations in glucan, water dikinase affect starch degradation and gametophore development in the moss Physcomitrella patens', Scientific Reports, vol. 9, art. 15114, pp. 1-13.en_ZA
dc.identifier.issn2045-2322 (online)
dc.identifier.other10.1038/s41598-019-51632-9
dc.identifier.urihttp://hdl.handle.net/2263/73009
dc.language.isoenen_ZA
dc.publisherNature Publishing Groupen_ZA
dc.rights© The Author(s) 2019. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License.en_ZA
dc.subjectStarch degradationen_ZA
dc.subjectPlantsen_ZA
dc.subjectPhyscomitrella patensen_ZA
dc.subjectGlucan, water dikinase (GWD)en_ZA
dc.titleMutations in glucan, water dikinase affect starch degradation and gametophore development in the moss Physcomitrella patensen_ZA
dc.typeArticleen_ZA

Files

Original bundle

Now showing 1 - 2 of 2
Loading...
Thumbnail Image
Name:
Mdodana_Mutations_2019.pdf
Size:
2.16 MB
Format:
Adobe Portable Document Format
Description:
Article
Loading...
Thumbnail Image
Name:
Mdodana_MutationsSuppl_2019.pdf
Size:
2.33 MB
Format:
Adobe Portable Document Format
Description:
Supplementary Material

License bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
license.txt
Size:
1.75 KB
Format:
Item-specific license agreed upon to submission
Description: