Mutations in glucan, water dikinase affect starch degradation and gametophore development in the moss Physcomitrella patens
dc.contributor.author | Mdodana, Ntombizanele T. | |
dc.contributor.author | Jewell, Jonathan F. | |
dc.contributor.author | Phiri, Ethel E. | |
dc.contributor.author | Smith, Marthinus L. | |
dc.contributor.author | Oberlander, Kenneth Carl | |
dc.contributor.author | Mahmoodi, S. | |
dc.contributor.author | Kossmann, J. | |
dc.contributor.author | Lloyd, J.R. | |
dc.date.accessioned | 2020-01-29T08:48:30Z | |
dc.date.available | 2020-01-29T08:48:30Z | |
dc.date.issued | 2019-12 | |
dc.description.abstract | The role of starch degradation in non-vascular plants is poorly understood. To expand our knowledge of this area, we have studied this process in Physcomitrella patens. This has been achieved through examination of the step known to initiate starch degradation in angiosperms, glucan phosphorylation, catalysed by glucan, water dikinase (GWD) enzymes. Phylogenetic analysis indicates that GWD isoforms can be divided into two clades, one of which contains GWD1/GWD2 and the other GWD3 isoforms. These clades split at a very early stage within plant evolution, as distinct sequences that cluster within each were identified in all major plant lineages. Of the five genes we identified within the Physcomitrella genome that encode GWD-like enzymes, two group within the GWD1/GWD2 clade and the others within the GWD3 clade. Proteins encoded by both loci in the GWD1/GWD2 clade, named PpGWDa and PpGWDb, are localised in plastids. Mutations of either PpGWDa or PpGWDb reduce starch phosphate abundance, however, a mutation at the PpGWDa locus had a much greater influence than one at PpGWDb. Only mutations affecting PpGWDa inhibited starch degradation. Mutants lacking this enzyme also failed to develop gametophores, a phenotype that could be chemically complemented using glucose supplementation within the growth medium. | en_ZA |
dc.description.department | Plant Production and Soil Science | en_ZA |
dc.description.librarian | am2020 | en_ZA |
dc.description.sponsorship | The Swiss/South African Joint Research Program grant 87391 and the NRF SARCHI chair “Genetic Tailoring of Biopolymers”. Bursary funding was obtained from the South African National Research Foundation for Ms Mdodana (SFH13091440165) and Mr Jewell (SFH14072881440). | en_ZA |
dc.description.uri | http://www.nature.com/srep | en_ZA |
dc.identifier.citation | Mdodana, N.T., Jewell, J.F., Phiri, E.E. et al. 2019, 'Mutations in glucan, water dikinase affect starch degradation and gametophore development in the moss Physcomitrella patens', Scientific Reports, vol. 9, art. 15114, pp. 1-13. | en_ZA |
dc.identifier.issn | 2045-2322 (online) | |
dc.identifier.other | 10.1038/s41598-019-51632-9 | |
dc.identifier.uri | http://hdl.handle.net/2263/73009 | |
dc.language.iso | en | en_ZA |
dc.publisher | Nature Publishing Group | en_ZA |
dc.rights | © The Author(s) 2019. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License. | en_ZA |
dc.subject | Starch degradation | en_ZA |
dc.subject | Plants | en_ZA |
dc.subject | Physcomitrella patens | en_ZA |
dc.subject | Glucan, water dikinase (GWD) | en_ZA |
dc.title | Mutations in glucan, water dikinase affect starch degradation and gametophore development in the moss Physcomitrella patens | en_ZA |
dc.type | Article | en_ZA |
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