Additional file 1: Figure S1. Seedling germination and development
of recalcitrant Cape Oxalis, where foliar leaf development and growth is
followed by delayed radicle growth. O. clavifolia Sond. (a) and O. glabra
Thunb. (b) one (i), three (ii), five (iii) and 10 (iv) days after germination. All
seedlings oriented with radicle pointing to bottom of figure. CT =
cotyledons, FL = foliar leaf, RD = radicle.
Additional file 2: Figure S2. Phylogenetic consensus tree constructed
with universal 16S region sequences for endophytic bacteria isolated
from Cape Oxalis (boldface font) and representative GenBank BLAST
results. Colour boxes indicate the most likely species identifications of
Oxalis isolates. B. megaterium and B. aryabhattai that had unresolved
relationships based on the consensus tree.
Additional file 3: Figure S3. Original red-and-green confocal staining
images of cross sections of sterilized Oxalis seeds indicating bacteria
(bright green rods) inside cavities. (i-ii) O. hirta, (iii) O. pes-caprae.
Additional file 4: Table S1. Properties of bacterial endophytes isolated
from Oxalis host plants, as described in literature [57–67].
Additional file 5: Table S2. A key to all species names relating to Fig. 2.