Evaluation of a virus neutralisation test for detection of Rift Valley fever antibodies in Suid Sera

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dc.contributor.author Lubisi, Baratang Alison
dc.contributor.author Ndouvhada, Phumudzo N.
dc.contributor.author Neiffer, Donald
dc.contributor.author Penrith, Mary-Louise
dc.contributor.author Sibanda, Donald-Ray
dc.contributor.author Bastos, Armanda D.S.
dc.date.accessioned 2019-12-02T06:48:44Z
dc.date.available 2019-12-02T06:48:44Z
dc.date.issued 2019-03-25
dc.description.abstract Rift Valley fever (RVF) is a vector-borne viral disease of ruminants mainly, and man, characterized by abortions and neonatal deaths in animals and flu-like to more severe symptoms that can result in death in humans. The disease is endemic in Africa, Saudi Arabia and Yemen, and outbreaks occur following proliferation of RVF virus (RVFV) infected mosquito vectors. Vertebrate animal maintenance hosts of RVFV, which serve as a source of virus during inter-epidemic periods remain unknown, with wild and domestic suids being largely overlooked. To address this, we evaluated the virus neutralization test (VNT) for RVF antibody detection in suid sera, as a first step in assessing the role of suids in the epidemiology of RVF in Africa. Testing of experimental and field sera from domestic pigs and warthogs with a commercial RVF competitive antibody ELISA, served as a reference standard against which the VNT results were compared. Results indicate that VNT can detect anti-RVFV antibodies within three days post-infection, has an analytical specificity of 100% and diagnostic sensitivity and specificity of 80% and 97%, respectively. Although labour-intensive and time-consuming, the VNT proved suitable for screening suid sera and plasma for presence of RVFV antibodies in viraemic and recovered animals. en_ZA
dc.description.department Mammal Research Institute en_ZA
dc.description.department Paraclinical Sciences en_ZA
dc.description.department Veterinary Tropical Diseases en_ZA
dc.description.department Zoology and Entomology en_ZA
dc.description.librarian am2019 en_ZA
dc.description.sponsorship The Economic Competitive Support Programme (ECSP) of the South African National Treasury, Gauteng Department of Agriculture and Rural Development (GDARD) and Joy Liebenberg Trust, and the APC was funded by the Agricultural Research Council. en_ZA
dc.description.uri http://www.mdpi.com/journal/tropicalmed en_ZA
dc.identifier.citation Lubisi, B.A., Ndouvhada, P.N., Neiffer, D. et al. 2019, 'Evaluation of a virus neutralisation test for detection of Rift Valley fever antibodies in Suid Sera', Tropical Medicine and Infectious Disease, vol. 4, art. 52, pp. 1-9. en_ZA
dc.identifier.issn 2414-6366 (online)
dc.identifier.other 10.3390/tropicalmed4010052
dc.identifier.uri http://hdl.handle.net/2263/72448
dc.language.iso en en_ZA
dc.publisher MDPI Publishing en_ZA
dc.rights © 2019 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license. en_ZA
dc.subject Inter-epidemic period en_ZA
dc.subject Domestic pig en_ZA
dc.subject Rift Valley fever virus (RVFV) en_ZA
dc.subject Rift Valley fever (RVF) en_ZA
dc.subject Virus neutralization test (VNT) en_ZA
dc.subject Enzyme-linked immunosorbent assay (ELISA) en_ZA
dc.title Evaluation of a virus neutralisation test for detection of Rift Valley fever antibodies in Suid Sera en_ZA
dc.type Article en_ZA


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