Evaluation of a virus neutralisation test for detection of Rift Valley fever antibodies in Suid Sera

dc.contributor.authorLubisi, Baratang Alison
dc.contributor.authorNdouvhada, Phumudzo N.
dc.contributor.authorNeiffer, Donald
dc.contributor.authorPenrith, Mary-Louise
dc.contributor.authorSibanda, Donald Ray
dc.contributor.authorBastos, Armanda D.S.
dc.contributor.emailamanda.bastos@up.ac.zaen_ZA
dc.date.accessioned2019-12-02T06:48:44Z
dc.date.available2019-12-02T06:48:44Z
dc.date.issued2019-03-25
dc.description.abstractRift Valley fever (RVF) is a vector-borne viral disease of ruminants mainly, and man, characterized by abortions and neonatal deaths in animals and flu-like to more severe symptoms that can result in death in humans. The disease is endemic in Africa, Saudi Arabia and Yemen, and outbreaks occur following proliferation of RVF virus (RVFV) infected mosquito vectors. Vertebrate animal maintenance hosts of RVFV, which serve as a source of virus during inter-epidemic periods remain unknown, with wild and domestic suids being largely overlooked. To address this, we evaluated the virus neutralization test (VNT) for RVF antibody detection in suid sera, as a first step in assessing the role of suids in the epidemiology of RVF in Africa. Testing of experimental and field sera from domestic pigs and warthogs with a commercial RVF competitive antibody ELISA, served as a reference standard against which the VNT results were compared. Results indicate that VNT can detect anti-RVFV antibodies within three days post-infection, has an analytical specificity of 100% and diagnostic sensitivity and specificity of 80% and 97%, respectively. Although labour-intensive and time-consuming, the VNT proved suitable for screening suid sera and plasma for presence of RVFV antibodies in viraemic and recovered animals.en_ZA
dc.description.departmentMammal Research Instituteen_ZA
dc.description.departmentParaclinical Sciencesen_ZA
dc.description.departmentVeterinary Tropical Diseasesen_ZA
dc.description.departmentZoology and Entomologyen_ZA
dc.description.librarianam2019en_ZA
dc.description.sponsorshipThe Economic Competitive Support Programme (ECSP) of the South African National Treasury, Gauteng Department of Agriculture and Rural Development (GDARD) and Joy Liebenberg Trust, and the APC was funded by the Agricultural Research Council.en_ZA
dc.description.urihttp://www.mdpi.com/journal/tropicalmeden_ZA
dc.identifier.citationLubisi, B.A., Ndouvhada, P.N., Neiffer, D. et al. 2019, 'Evaluation of a virus neutralisation test for detection of Rift Valley fever antibodies in Suid Sera', Tropical Medicine and Infectious Disease, vol. 4, art. 52, pp. 1-9.en_ZA
dc.identifier.issn2414-6366 (online)
dc.identifier.other10.3390/tropicalmed4010052
dc.identifier.urihttp://hdl.handle.net/2263/72448
dc.language.isoenen_ZA
dc.publisherMDPI Publishingen_ZA
dc.rights© 2019 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.en_ZA
dc.subjectInter-epidemic perioden_ZA
dc.subjectDomestic pig (Sus domesticus) en_ZA
dc.subjectRift Valley fever virus (RVFV)en_ZA
dc.subjectRift Valley fever (RVF)en_ZA
dc.subjectVirus neutralization test (VNT)en_ZA
dc.subjectEnzyme-linked immunosorbent assay (ELISA)en_ZA
dc.titleEvaluation of a virus neutralisation test for detection of Rift Valley fever antibodies in Suid Seraen_ZA
dc.typeArticleen_ZA

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