dc.contributor.author |
Ragan, Izabela K.
|
|
dc.contributor.author |
Schuck, Kaitlynn N.
|
|
dc.contributor.author |
Upreti, Deepa
|
|
dc.contributor.author |
Odendaal, Lieza
|
|
dc.contributor.author |
Richt, Juergen A.
|
|
dc.contributor.author |
Trujillo, Jessie D.
|
|
dc.contributor.author |
Wilson, William C.
|
|
dc.contributor.author |
Davis, A.S. (Sally)
|
|
dc.date.accessioned |
2019-07-30T08:44:03Z |
|
dc.date.issued |
2019-06 |
|
dc.description |
This work was done in partial completion of Izabela Ragan’s PhD dissertation. |
en_ZA |
dc.description.abstract |
Sporadic outbreaks of Rift Valley fever virus (RVFV), a zoonotic, mosquito-borne Phlebovirus, cause abortion storms and death in sheep and cattle resulting in catastrophic economic impacts in endemic regions of Africa. More recently, with changes in competent vector distribution, growing international trade, and its potential use for bioterrorism, RVFV has become a transboundary animal disease of significant concern. New and sensitive techniques that determine RVFV presence, while lessening the potential for environmental contamination and human risk, through the use of inactivated, noninfectious samples such as formalin-fixed, paraffin-embedded (FFPE) tissues are needed. FFPE tissue in situ hybridization (ISH) enables the detection of nucleic acid sequences within the visual context of cellular and tissue morphology. Here, we present a chromogenic pan-RVFV ISH assay based on RNAscope® technology, which is able to detect multiple RVFV strains in FFPE tissues, enabling visual correlation of RVFV RNA presence with histopathologic lesions. |
en_ZA |
dc.description.department |
Paraclinical Sciences |
en_ZA |
dc.description.embargo |
2020-06-26 |
|
dc.description.librarian |
hj2019 |
en_ZA |
dc.description.sponsorship |
The USDA Agricultural Research Service and the Department of Diagnostic Medicine, College of Veterinary Medicine, Kansas State University’s startup funds for Dr. Davis. This work was also supported through the Department of Homeland Security (grant no. 2010-ST061-AG0001), Center of Excellence for Emerging and Zoonotic Animal Diseases (CEEZAD). |
en_ZA |
dc.description.uri |
http://online.liebertpub.com/VBZ |
en_ZA |
dc.identifier.citation |
Ragan, I.K., Schuck, K.N., Upreti, D. et al. 2019, 'Rift Valley fever viral RNA detection by in situ hybridization in formalin-fixed, paraffin-embedded tissues', Vector Borne and Zoonotic Diseases, vol. 19, no. 7, pp. 553-556. |
en_ZA |
dc.identifier.issn |
1530-3667 (print) |
|
dc.identifier.issn |
1557-7759 (online) |
|
dc.identifier.other |
10.1089/vbz.2018.2383 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/70820 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
Mary Ann Liebert |
en_ZA |
dc.rights |
© 2019 Mary Ann Liebert, Inc. All rights reserved. |
en_ZA |
dc.subject |
Rift Valley fever virus (RVFV) |
en_ZA |
dc.subject |
Formalin-fixed, paraffin-embedded (FFPE) |
en_ZA |
dc.subject |
In situ hybridization (ISH) |
en_ZA |
dc.title |
Rift Valley fever viral RNA detection by in situ hybridization in formalin-fixed, paraffin-embedded tissues |
en_ZA |
dc.type |
Postprint Article |
en_ZA |