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dc.contributor.author | Chermprapai, Suttiwee![]() |
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dc.contributor.author | Broere, Femke![]() |
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dc.contributor.author | Schlotter, Yvette M.![]() |
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dc.contributor.author | Veldhuizen, Edwin J.A.![]() |
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dc.contributor.author | Rutten, Victor P.M.G.![]() |
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dc.date.accessioned | 2019-06-12T09:43:23Z | |
dc.date.available | 2019-06-12T09:43:23Z | |
dc.date.issued | 2018-12 | |
dc.description.abstract | Keratinocytes (KC) are the main cellular components of the stratum corneum that constitutes a solid physical skin barrier representing the first line of defense against pathogens. Moreover, KC are potent producers of inflammatory mediators and antimicrobial peptides (AMP) when activated through their pattern recognition receptors. In atopic dermatitis (AD) the protective skin barrier may be compromised due to barrier disruption, secondary infection and accelerated secretion of inflammatory cytokines which may also affect AMP expression in the skin. In the present study, we addressed the responses of a canine KC cell line upon exposure to Staphylococcus pseudintermedius, typically found on canine atopic skin during secondary infections, and stimulation by individual AD-associated ligands and cytokines. All stimuli induced a significant increase in expression of the pro-inflammatory cytokine genes tumor necrosis factor (TNF)-α and interleukin (IL)-8, but with different kinetics. Limited effects were observed on AMP gene expression except for K9CATH which was significantly upregulated upon bacterial infection but with none of the individual AD-associated ligands. Interestingly, K9CATH possessed antimicrobial activity towards Staphylococcus pseudintermedius, indicating that K9CATH expression is a specific defense reaction towards bacterial infection and not part of a general pro-inflammatory profile of KC. | en_ZA |
dc.description.department | Veterinary Tropical Diseases | en_ZA |
dc.description.librarian | am2019 | en_ZA |
dc.description.sponsorship | We would like to acknowledge Koji Nishifuji for providing the canine keratinocyte cell line (MSCEK) and Hans J.C.M. Vernooij for his statistical analyses advice. This study was supported by a scholarship from Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand. | en_ZA |
dc.description.sponsorship | A scholarship from Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand. | en_ZA |
dc.description.uri | http://www.elsevier.com/locate/vetimm | en_ZA |
dc.identifier.citation | Chermprapai, S., Broere, F., Schlotter, Y.M. et al. 2018, 'A canine keratinocyte cell line expresses antimicrobial peptide and cytokine genes upon stimulation with bacteria, microbial ligands and recombinant cytokines', Veterinary Immunology and Immunopathology, vol. 206, pp. 35-40. | en_ZA |
dc.identifier.issn | 0165-2427 (print) | |
dc.identifier.issn | 1873-2534 (online) | |
dc.identifier.other | 10.1016/j.vetimm.2018.11.009 | |
dc.identifier.uri | http://hdl.handle.net/2263/70157 | |
dc.language.iso | en | en_ZA |
dc.publisher | Elsevier | en_ZA |
dc.rights | © 2018 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/). | en_ZA |
dc.subject | β-Defensin | en_ZA |
dc.subject | Cathelicidin | en_ZA |
dc.subject | Pattern recognition receptor | en_ZA |
dc.subject | Canine | en_ZA |
dc.subject | Dogs (Canis familiaris) | en_ZA |
dc.subject | Activation | en_ZA |
dc.subject | Atopic dermatitis (AD) | en_ZA |
dc.subject | Keratinocytes (KC) | en_ZA |
dc.subject | Inflammatory mediators | en_ZA |
dc.subject | Antimicrobial peptides (AMP) | en_ZA |
dc.title | A canine keratinocyte cell line expresses antimicrobial peptide and cytokine genes upon stimulation with bacteria, microbial ligands and recombinant cytokines | en_ZA |
dc.type | Article | en_ZA |