Serum protein electrophoresis in healthy and injured southern white rhinoceros (Ceratotherium simum simum)
Loading...
Date
Authors
Hooijberg, Emma Henriette
Miller, Michele
Cray, Carolyn
Buss, Peter Erik
Steenkamp, Gerhardus
Goddard, Amelia
Journal Title
Journal ISSN
Volume Title
Publisher
Public Library of Science
Abstract
Investigation of globulin fractions by serum protein electrophoresis (SPE) is the first step
towards evaluation of the proteome in the southern white rhinoceros (Ceratotherium simum
simum). Furthermore, identification of changes in globulins in animals with poaching and
other injuries can guide discovery of potentially useful biomarkers of inflammation. The aim
of this study was to develop reference intervals for agarose gel SPE in healthy white rhinoceros
and to compare these serum protein electrophoresis results to those from animals with
tissue trauma. Reference intervals for total serum protein and agarose gel electrophoretic
albumin and globulin fractions were generated using serum samples from 49 healthy freeranging
adult white rhinoceros. A standardised gating system together with identification of
specific proteins by mass spectrometry aided in fraction identification. Six globulin fractions
were identified: α1a, α1b, α2, β1, β2 and γ. Reference intervals were generated for total
serum protein (76±111 g/L), albumin (10±27 g/L) and globulin fractions (α1a: 1.6±3.2 g/L;
α1b: 1.7±3.6 g/L; α2: 16.1±26.6 g/L; β1: 6.6±18.2 g/L; β2: 11.8±30.4 g/L; γ: 10.4±23.1 g/L;
albumin: globulin ratio: 0.12±0.39). Results were compared to those from 30 animals with
various degrees and chronicities of tissue trauma. Wounded animals had lower concentrations
of total serum protein, albumin, total globulin, α and β1 globulins, lower percentages of
α2 and β1 globulins, and higher percentages of β2 and γ globulins. These protein changes
are similar to those seen in human patients with wounds rather than classic acute phase or
chronic inflammatory responses.
Description
S1 Text. Methods. Detailed liquid chromatography±mass spectrometry method description.
S1 Table. Rhinoceros data. Raw SPE data and clinical information for healthy and injured rhinoceros.
S2 Table. Proteomic results. Detailed information for the proteins identified in the alpha1b and alpha2 bands.
S1 Table. Rhinoceros data. Raw SPE data and clinical information for healthy and injured rhinoceros.
S2 Table. Proteomic results. Detailed information for the proteins identified in the alpha1b and alpha2 bands.
Keywords
Globulin fractions, Serum samples, Albumin, Alpha 1a globulin, Alpha 1b globulin, Alpha 2 globulin, Beta globulin, Immunoglobulin, Plasma protein, Proteome, Unclassified drug, Agar gel electrophoresis, Blood sampling, Chronicity, Ecological procedures, Inflammation, Injury severity, Mass spectrometry, Placental mammal, Poaching, Protein blood level, Protein electrophoresis, Proteomics, Standardization, Tissue injury, White rhinoceros (Ceratotherium simum), Serum protein electrophoresis (SPE)
Sustainable Development Goals
Citation
Hooijberg EH, Miller M, Cray C, Buss P,
Steenkamp G, Goddard A (2018) Serum protein
electrophoresis in healthy and injured southern
white rhinoceros (Ceratotherium simum simum).
PLoS ONE 13(7): e0200347. https://DOI.org/10.1371/journal.pone.0200347.