Investigation of globulin fractions by serum protein electrophoresis (SPE) is the first step
towards evaluation of the proteome in the southern white rhinoceros (Ceratotherium simum
simum). Furthermore, identification of changes in globulins in animals with poaching and
other injuries can guide discovery of potentially useful biomarkers of inflammation. The aim
of this study was to develop reference intervals for agarose gel SPE in healthy white rhinoceros
and to compare these serum protein electrophoresis results to those from animals with
tissue trauma. Reference intervals for total serum protein and agarose gel electrophoretic
albumin and globulin fractions were generated using serum samples from 49 healthy freeranging
adult white rhinoceros. A standardised gating system together with identification of
specific proteins by mass spectrometry aided in fraction identification. Six globulin fractions
were identified: α1a, α1b, α2, β1, β2 and γ. Reference intervals were generated for total
serum protein (76±111 g/L), albumin (10±27 g/L) and globulin fractions (α1a: 1.6±3.2 g/L;
α1b: 1.7±3.6 g/L; α2: 16.1±26.6 g/L; β1: 6.6±18.2 g/L; β2: 11.8±30.4 g/L; γ: 10.4±23.1 g/L;
albumin: globulin ratio: 0.12±0.39). Results were compared to those from 30 animals with
various degrees and chronicities of tissue trauma. Wounded animals had lower concentrations
of total serum protein, albumin, total globulin, α and β1 globulins, lower percentages of
α2 and β1 globulins, and higher percentages of β2 and γ globulins. These protein changes
are similar to those seen in human patients with wounds rather than classic acute phase or
chronic inflammatory responses.
Pneumococcal polysaccharide vaccines (PPVs) and conjugate vaccines (PCVs), of which PPV23 and PCV13 are the current front runners, have had a significant, beneficial impact on public health. With regard to PPV23, there has ...
Senekal, Frederick Petrus(University of Pretoria, 2009-04-08)
The protein folding problem is examined. Specifically, the problem of predicting protein secondary structure from the amino acid sequence is investigated. A literature study is presented into the protein folding process ...
Joubert, Yolandi(University of Pretoria, 2008-08-14)
Malaria is the cause of over one million deaths per year, primarily in African children. The parasite responsible for the most virulent form of malaria, is Plasmodium falciparum. Protein structure plays a pivotal role in ...