Clofazimine exposure in vitro selects efflux pump mutants and bedaquiline resistance

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dc.contributor.author Ismail, Nabila
dc.contributor.author Peters, Remco P.H.
dc.contributor.author Ismail, Nazir A.
dc.contributor.author Omar, Shaheed V.
dc.date.accessioned 2019-03-08T07:41:27Z
dc.date.issued 2019-03
dc.description.abstract Six in vitro clofazimine-resistant spontaneous mutants obtained from a wild-type or pyrazinamide-resistant ATCC reference strain were selected to evaluate bedaquiline cross-resistance. The reverse was conducted for bedaquiline mutants. All clofazimine mutants harboring an rv0678 mutation displayed phenotypic cross-resistance. We observed the same for rv0678 bedaquiline mutants; however, atpE bedaquiline mutants showed no phenotypic cross-resistance. This confirms that upfront clofazimine usage may impact subsequent bedaquiline use due to a shared efflux resistance pathway. en_ZA
dc.description.department Medical Microbiology en_ZA
dc.description.embargo 2019-09-01
dc.description.librarian hj2019 en_ZA
dc.description.sponsorship N.I. was supported by the National Research Fund (SFH150723130071) and theUniversityof Pretoria.This project was funded by the WHO Supranational Reference TB Laboratory,National Institute for Communicable Diseases, Johannesburg, South Africa, and by theNational Health Laboratory Services (NHLS) Research Trust (grant 004_94640). en_ZA
dc.description.uri http://aac.asm.org en_ZA
dc.identifier.citation Ismail, N., Peters, R.P.H., Ismail, N.A. et al. 2019, Clofazimine exposure in vitro selects efflux pump mutants and bedaquiline resistance', Antimicrobial Agents and Chemotherapy, vol. 63, no. 1, art. 02141-18, pp. 1-4. en_ZA
dc.identifier.issn 0066-4804 (print)
dc.identifier.issn 1098-6596 (online)
dc.identifier.other 10.1128/AAC.02141-18
dc.identifier.uri http://hdl.handle.net/2263/68613
dc.language.iso en en_ZA
dc.publisher American Society for Microbiology en_ZA
dc.rights © 2019 American Society forMicrobiology. All Rights Reserved. en_ZA
dc.subject Bedaquiline en_ZA
dc.subject Clofazimine en_ZA
dc.subject Cross-resistance en_ZA
dc.subject Efflux pump mutants en_ZA
dc.subject Drug-resistant tuberculosis (DR-TB) en_ZA
dc.title Clofazimine exposure in vitro selects efflux pump mutants and bedaquiline resistance en_ZA
dc.type Postprint Article en_ZA


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