Clofazimine exposure in vitro selects efflux pump mutants and bedaquiline resistance
| dc.contributor.author | Ismail, Nabila | |
| dc.contributor.author | Peters, Remco P.H. | |
| dc.contributor.author | Ismail, Nazir Ahmed | |
| dc.contributor.author | Omar, Shaheed Vally | |
| dc.date.accessioned | 2019-03-08T07:41:27Z | |
| dc.date.issued | 2019-03 | |
| dc.description.abstract | Six in vitro clofazimine-resistant spontaneous mutants obtained from a wild-type or pyrazinamide-resistant ATCC reference strain were selected to evaluate bedaquiline cross-resistance. The reverse was conducted for bedaquiline mutants. All clofazimine mutants harboring an rv0678 mutation displayed phenotypic cross-resistance. We observed the same for rv0678 bedaquiline mutants; however, atpE bedaquiline mutants showed no phenotypic cross-resistance. This confirms that upfront clofazimine usage may impact subsequent bedaquiline use due to a shared efflux resistance pathway. | en_ZA |
| dc.description.department | Medical Microbiology | en_ZA |
| dc.description.embargo | 2019-09-01 | |
| dc.description.librarian | hj2019 | en_ZA |
| dc.description.sponsorship | N.I. was supported by the National Research Fund (SFH150723130071) and theUniversityof Pretoria.This project was funded by the WHO Supranational Reference TB Laboratory,National Institute for Communicable Diseases, Johannesburg, South Africa, and by theNational Health Laboratory Services (NHLS) Research Trust (grant 004_94640). | en_ZA |
| dc.description.uri | http://aac.asm.org | en_ZA |
| dc.identifier.citation | Ismail, N., Peters, R.P.H., Ismail, N.A. et al. 2019, Clofazimine exposure in vitro selects efflux pump mutants and bedaquiline resistance', Antimicrobial Agents and Chemotherapy, vol. 63, no. 1, art. 02141-18, pp. 1-4. | en_ZA |
| dc.identifier.issn | 0066-4804 (print) | |
| dc.identifier.issn | 1098-6596 (online) | |
| dc.identifier.other | 10.1128/AAC.02141-18 | |
| dc.identifier.uri | http://hdl.handle.net/2263/68613 | |
| dc.language.iso | en | en_ZA |
| dc.publisher | American Society for Microbiology | en_ZA |
| dc.rights | © 2019 American Society forMicrobiology. All Rights Reserved. | en_ZA |
| dc.subject | Bedaquiline | en_ZA |
| dc.subject | Clofazimine | en_ZA |
| dc.subject | Cross-resistance | en_ZA |
| dc.subject | Efflux pump mutants | en_ZA |
| dc.subject | Drug-resistant tuberculosis (DR-TB) | en_ZA |
| dc.title | Clofazimine exposure in vitro selects efflux pump mutants and bedaquiline resistance | en_ZA |
| dc.type | Postprint Article | en_ZA |