In vitro and in vivo activity of Myrsine africana on elastase inhibition and anti-wrinkle activity

Show simple item record Lall, Namrita Kishore, Navneet Fibrich, Bianca Lambrechts, Isa Anina 2018-08-22T05:45:04Z 2018-08-22T05:45:04Z 2017-11-13
dc.description.abstract BACKGROUND : Myrsine africana (MA) is a plant traditionally used in South Africa to treat various diseases. OBJECTIVE : The ethanolic extract of MA, was used for in vitro and in vivo studies to determine its elastase inhibitory activity. MATERIALS AND METHODS : MA and its isolated compound, myrsinoside B, were tested in vitro for their elastase inhibitory activity. The MA extract was also evaluated for mutagenicity using two strains of Salmonella typhimurium (TA 98 and TA 100), microbial count, metal analysis, and stability. In vivo studies included irritancy and wrinkle reduction trials using Visioscan and Visioface. RESULTS : The leaf extract showed good elastase inhibition with a 50% inhibitory concentration (IC50) of 28.04 µg/ml. Myrsinoside B inhibited the elastase enzyme at an IC50 of 4.68 ± 0.34 μg/ml. No colony growth observed during mutagenicity studies and it was concluded that MA ethanolic extract is a nonmutagen. MA extract was found to be a nonirritant during the patch test clinical trial. MA was found to contain negligible amounts of microorganisms and heavy metals. Gel cream containing MA crude extract was found to be stable for 2 years when kept at temperatures below 30°C. In clinical trials (in vivo), it was found that the test product containing 5% ethanolic extract of MA was effective in reducing wrinkles after application 2 times a day for 14 days and 28 days compared to the placebo aqueous cream. CONCLUSION : MA is effective in reducing the appearance of wrinkles. en_ZA
dc.description.department Plant Production and Soil Science en_ZA
dc.description.librarian am2018 en_ZA
dc.description.uri en_ZA
dc.identifier.citation Lall N, Kishore N, Fibrich B, Lambrechts IA. In vitro and In vivo activity of Myrsine africana on elastase inhibition and anti-wrinkle activity. Pharmacognosy Magazine 2017;13:583-9. en_ZA
dc.identifier.issn 0973-1296 (print)
dc.identifier.issn 0976-4062 (online)
dc.identifier.other 10.4103/pm.pm_145_17
dc.language.iso en en_ZA
dc.publisher Medknow Publications en_ZA
dc.rights © 2017 Pharmacognosy Magazine. This is an open access article distributed under the terms of the Creative Commons Attribution‑NonCommercial‑ShareAlike 3.0 License. en_ZA
dc.subject Antiwrinkle en_ZA
dc.subject Elastase en_ZA
dc.subject Myrsinoside B en_ZA
dc.subject Myrsine africana (MA) en_ZA
dc.title In vitro and in vivo activity of Myrsine africana on elastase inhibition and anti-wrinkle activity en_ZA
dc.type Article en_ZA

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