In vitro and in vivo activity of Myrsine africana on elastase inhibition and anti-wrinkle activity

dc.contributor.authorLall, Namrita
dc.contributor.authorKishore, Navneet
dc.contributor.authorFibrich, Bianca
dc.contributor.authorLambrechts, Isa Anina
dc.contributor.emailnamrita.lall@up.ac.zaen_ZA
dc.date.accessioned2018-08-22T05:45:04Z
dc.date.available2018-08-22T05:45:04Z
dc.date.issued2017-11-13
dc.description.abstractBACKGROUND : Myrsine africana (MA) is a plant traditionally used in South Africa to treat various diseases. OBJECTIVE : The ethanolic extract of MA, was used for in vitro and in vivo studies to determine its elastase inhibitory activity. MATERIALS AND METHODS : MA and its isolated compound, myrsinoside B, were tested in vitro for their elastase inhibitory activity. The MA extract was also evaluated for mutagenicity using two strains of Salmonella typhimurium (TA 98 and TA 100), microbial count, metal analysis, and stability. In vivo studies included irritancy and wrinkle reduction trials using Visioscan and Visioface. RESULTS : The leaf extract showed good elastase inhibition with a 50% inhibitory concentration (IC50) of 28.04 µg/ml. Myrsinoside B inhibited the elastase enzyme at an IC50 of 4.68 ± 0.34 μg/ml. No colony growth observed during mutagenicity studies and it was concluded that MA ethanolic extract is a nonmutagen. MA extract was found to be a nonirritant during the patch test clinical trial. MA was found to contain negligible amounts of microorganisms and heavy metals. Gel cream containing MA crude extract was found to be stable for 2 years when kept at temperatures below 30°C. In clinical trials (in vivo), it was found that the test product containing 5% ethanolic extract of MA was effective in reducing wrinkles after application 2 times a day for 14 days and 28 days compared to the placebo aqueous cream. CONCLUSION : MA is effective in reducing the appearance of wrinkles.en_ZA
dc.description.departmentPlant Production and Soil Scienceen_ZA
dc.description.librarianam2018en_ZA
dc.description.urihttp://www.phcog.comen_ZA
dc.identifier.citationLall N, Kishore N, Fibrich B, Lambrechts IA. In vitro and In vivo activity of Myrsine africana on elastase inhibition and anti-wrinkle activity. Pharmacognosy Magazine 2017;13:583-9.en_ZA
dc.identifier.issn0973-1296 (print)
dc.identifier.issn0976-4062 (online)
dc.identifier.other10.4103/pm.pm_145_17
dc.identifier.urihttp://hdl.handle.net/2263/66307
dc.language.isoenen_ZA
dc.publisherMedknow Publicationsen_ZA
dc.rights© 2017 Pharmacognosy Magazine. This is an open access article distributed under the terms of the Creative Commons Attribution‑NonCommercial‑ShareAlike 3.0 License.en_ZA
dc.subjectAntiwrinkleen_ZA
dc.subjectElastaseen_ZA
dc.subjectMyrsinoside Ben_ZA
dc.subjectMyrsine africana (MA)en_ZA
dc.titleIn vitro and in vivo activity of Myrsine africana on elastase inhibition and anti-wrinkle activityen_ZA
dc.typeArticleen_ZA

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Lall_InVitro_2017.pdf
Size:
499.75 KB
Format:
Adobe Portable Document Format
Description:
Article

License bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
license.txt
Size:
1.75 KB
Format:
Item-specific license agreed upon to submission
Description: