The population structure of clinical extra-intestinal Escherichia coli in a teaching hospital from Nigeria
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Date
Authors
Seni, Jeremiah
Peirano, Gisele
Okon, Kenneth Okwong
Jibrin, Yusuf Bara
Mohammed, Alkali
Mshana, Stephen E.
DeVinney, Rebekah
Pitout, Johann D.D.
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
Limited information is available regarding the population structure of extra-intestinal pathogenic Escherichia coli (ExPEC) in Africa. Antimicrobial resistance profiles, sequence types (STs) and fimH types were determined on 60 clinical ExPEC from Nigeria using a 7-single nucleotide polymorphism quantitative PCR and sequencing of certain genes. Different ST131 clades were identified with a multiplex PCR. The isolates were mostly obtained from urines (58.3%). Not-susceptibility rates were as follows: trimethoprim-sulfamethoxazole (98%), cefotaxime (68%), gentamicin (55%), ciprofloxacin (62%) and piperacillin-tazobactam (2%). Dominant STs were associated with CTX-M-15 and included ST131-fimH30 (23%), ST457-fimH145 (20%), ST405-fimH27 (13%) and ST95-fimH41 (10%). We found the 7-SNP qPCR to be simple and cost-effective that can be utilized to tract different ExPEC clones on a global scale. This study provided insight into the population structure of ExPEC from Nigeria showing high prevalence of the rarely reported ST457 and the presence of multidrug resistant ST95.
Description
Keywords
Escherichia coli, Population structure, Molecular typing, Nigeria, Nucleotide, Polymerase chain reaction (PCR), Antimicrobial, Trimethoprim-sulfamethoxazole, Gentamicin, Piperacillin-tazobactam, Ciprofloxacin, Cefotaxime, Bloodstream infections (BSIs), Gram-negative bacterium
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Citation
Seni, J., Peirano, G., Okon, K.O. et al. 2018, 'The population structure of clinical extra-intestinal Escherichia coli in a teaching hospital from Nigeria', Diagnostic Microbiology and Infectious Disease, vol. 92, no. 1, pp. 46-49.