The population structure of clinical extra-intestinal Escherichia coli in a teaching hospital from Nigeria

Show simple item record Seni, Jeremiah Peirano, Giselle Okon, Kenneth Okwong Jibrin, Yusuf Bara Mohammed, Alkali Mshana, Stephen E. DeVinney, Rebekah Pitout, Johann D.D. 2018-06-25T06:02:49Z 2018-09
dc.description.abstract Limited information is available regarding the population structure of extra-intestinal pathogenic Escherichia coli (ExPEC) in Africa. Antimicrobial resistance profiles, sequence types (STs) and fimH types were determined on 60 clinical ExPEC from Nigeria using a 7-single nucleotide polymorphism quantitative PCR and sequencing of certain genes. Different ST131 clades were identified with a multiplex PCR. The isolates were mostly obtained from urines (58.3%). Not-susceptibility rates were as follows: trimethoprim-sulfamethoxazole (98%), cefotaxime (68%), gentamicin (55%), ciprofloxacin (62%) and piperacillin-tazobactam (2%). Dominant STs were associated with CTX-M-15 and included ST131-fimH30 (23%), ST457-fimH145 (20%), ST405-fimH27 (13%) and ST95-fimH41 (10%). We found the 7-SNP qPCR to be simple and cost-effective that can be utilized to tract different ExPEC clones on a global scale. This study provided insight into the population structure of ExPEC from Nigeria showing high prevalence of the rarely reported ST457 and the presence of multidrug resistant ST95. en_ZA
dc.description.department Medical Microbiology en_ZA
dc.description.embargo 2019-09-01
dc.description.librarian hj2018 en_ZA
dc.description.sponsorship The University of Calgary (UCRG Seed Grant) to RD and a research grant from the Calgary Laboratory Services (#10015169) to JDDP. en_ZA
dc.description.uri en_ZA
dc.identifier.citation Seni, J., Peirano, G., Okon, K.O. et al. 2018, 'The population structure of clinical extra-intestinal Escherichia coli in a teaching hospital from Nigeria', Diagnostic Microbiology and Infectious Disease, vol. 92, no. 1, pp. 46-49. en_ZA
dc.identifier.issn 0732-8893 (print)
dc.identifier.issn 1879-0070 (online)
dc.identifier.other 10.1016/j.diagmicrobio.2018.04.001
dc.language.iso en en_ZA
dc.publisher Elsevier en_ZA
dc.rights © 2018 Elsevier Inc. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Diagnostic Microbiology and Infectious Diseases. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in Diagnostic Microbiology and Infectious Diseases, vol. 92, no. 1, pp. 46-49, 2018. doi : 10.1016/j.diagmicrobio.2018.04.001. en_ZA
dc.subject Escherichia coli en_ZA
dc.subject Population structure en_ZA
dc.subject Molecular typing en_ZA
dc.subject Nigeria en_ZA
dc.subject Nucleotide en_ZA
dc.subject Polymerase chain reaction (PCR) en_ZA
dc.subject Antimicrobial en_ZA
dc.subject Trimethoprim-sulfamethoxazole en_ZA
dc.subject Gentamicin en_ZA
dc.subject Piperacillin-tazobactam en_ZA
dc.subject Ciprofloxacin en_ZA
dc.subject Cefotaxime en_ZA
dc.subject Bloodstream infections en_ZA
dc.subject Gram-negative bacterium en_ZA
dc.title The population structure of clinical extra-intestinal Escherichia coli in a teaching hospital from Nigeria en_ZA
dc.type Postprint Article en_ZA

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