The population structure of clinical extra-intestinal Escherichia coli in a teaching hospital from Nigeria

dc.contributor.authorSeni, Jeremiah
dc.contributor.authorPeirano, Gisele
dc.contributor.authorOkon, Kenneth Okwong
dc.contributor.authorJibrin, Yusuf Bara
dc.contributor.authorMohammed, Alkali
dc.contributor.authorMshana, Stephen E.
dc.contributor.authorDeVinney, Rebekah
dc.contributor.authorPitout, Johann D.D.
dc.date.accessioned2018-06-25T06:02:49Z
dc.date.issued2018-09
dc.description.abstractLimited information is available regarding the population structure of extra-intestinal pathogenic Escherichia coli (ExPEC) in Africa. Antimicrobial resistance profiles, sequence types (STs) and fimH types were determined on 60 clinical ExPEC from Nigeria using a 7-single nucleotide polymorphism quantitative PCR and sequencing of certain genes. Different ST131 clades were identified with a multiplex PCR. The isolates were mostly obtained from urines (58.3%). Not-susceptibility rates were as follows: trimethoprim-sulfamethoxazole (98%), cefotaxime (68%), gentamicin (55%), ciprofloxacin (62%) and piperacillin-tazobactam (2%). Dominant STs were associated with CTX-M-15 and included ST131-fimH30 (23%), ST457-fimH145 (20%), ST405-fimH27 (13%) and ST95-fimH41 (10%). We found the 7-SNP qPCR to be simple and cost-effective that can be utilized to tract different ExPEC clones on a global scale. This study provided insight into the population structure of ExPEC from Nigeria showing high prevalence of the rarely reported ST457 and the presence of multidrug resistant ST95.en_ZA
dc.description.departmentMedical Microbiologyen_ZA
dc.description.embargo2019-09-01
dc.description.librarianhj2018en_ZA
dc.description.sponsorshipThe University of Calgary (UCRG Seed Grant) to RD and a research grant from the Calgary Laboratory Services (#10015169) to JDDP.en_ZA
dc.description.urihttp://www.elsevier.com/locate/diagmicrobioen_ZA
dc.identifier.citationSeni, J., Peirano, G., Okon, K.O. et al. 2018, 'The population structure of clinical extra-intestinal Escherichia coli in a teaching hospital from Nigeria', Diagnostic Microbiology and Infectious Disease, vol. 92, no. 1, pp. 46-49.en_ZA
dc.identifier.issn0732-8893 (print)
dc.identifier.issn1879-0070 (online)
dc.identifier.other10.1016/j.diagmicrobio.2018.04.001
dc.identifier.urihttp://hdl.handle.net/2263/65222
dc.language.isoenen_ZA
dc.publisherElsevieren_ZA
dc.rights© 2018 Elsevier Inc. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Diagnostic Microbiology and Infectious Diseases. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in Diagnostic Microbiology and Infectious Diseases, vol. 92, no. 1, pp. 46-49, 2018. doi : 10.1016/j.diagmicrobio.2018.04.001.en_ZA
dc.subjectEscherichia colien_ZA
dc.subjectPopulation structureen_ZA
dc.subjectMolecular typingen_ZA
dc.subjectNigeriaen_ZA
dc.subjectNucleotideen_ZA
dc.subjectPolymerase chain reaction (PCR)en_ZA
dc.subjectAntimicrobialen_ZA
dc.subjectTrimethoprim-sulfamethoxazoleen_ZA
dc.subjectGentamicinen_ZA
dc.subjectPiperacillin-tazobactamen_ZA
dc.subjectCiprofloxacinen_ZA
dc.subjectCefotaximeen_ZA
dc.subjectBloodstream infections (BSIs)en_ZA
dc.subjectGram-negative bacteriumen_ZA
dc.titleThe population structure of clinical extra-intestinal Escherichia coli in a teaching hospital from Nigeriaen_ZA
dc.typePostprint Articleen_ZA

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