Beneficial effects of folic acid on the kidneys and testes of adult albino rats after exposure to methomyl

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dc.contributor.author Sakr, Samar
dc.contributor.author Hassanien, Hanan
dc.contributor.author Bester, Megan Jean
dc.contributor.author Arbi, Sandra
dc.contributor.author Sobhy, Azza
dc.contributor.author El Negris, Heba
dc.contributor.author Steenkamp, Vanessa
dc.date.accessioned 2018-06-14T06:20:35Z
dc.date.issued 2018-03
dc.description.abstract The aim of this study was to evaluate the protective effect of folate against methomyl-induced toxicity on the kidneys and testes of male rats. Adult male albino rats were divided into four groups; Group I served as the control (vehicle), Group II received folic acid (1.1 mg per kg b.wt.), Group III methomyl (1 mg per kg b.wt.) and Group IV folic acid and methomyl. Treatments were administered via oral gavage on a daily basis for 14 weeks. Thereafter blood samples were collected and serum creatinine, testosterone and total antioxidant capacity (TAC) were determined. Animals were sacrificed and semen analysis was conducted. The kidneys and testes were excised and malondialdehyde (MDA) levels were determined. Histopathological and immunohistochemical analyses for caspase-3 were also undertaken. Methomyl treatment resulted in a significant (p < 0.001) elevation of creatinine and MDA levels and significant (p < 0.001) reduction in testosterone and TAC levels. Furthermore, methomyl caused a significant (p < 0.001) reduction in sperm quality. Histopathological examination indicated testicular and renal damage with strong immunoreactivity for caspase-3. Functional and tissue damage was prevented in rats treated with a combination of methomyl and folic acid. This is ascribed to the ability of folate to directly scavenge reactive oxygen species and indirectly enhance cellular redox homeostasis. This study identified that folic acid supplementation may have a beneficial effect in preventing or reducing the deleterious effects of methomyl exposure on kidney as well as testis structure and function. Future studies should focus on the fertility outcome/pregnancy index in rats. en_ZA
dc.description.department Anatomy en_ZA
dc.description.department Pharmacology en_ZA
dc.description.embargo 2019-03-15
dc.description.librarian hj2018 en_ZA
dc.description.uri http://rsc.li/toxicology-research en_ZA
dc.identifier.citation Sakr, S., Hassanien, H., Bester, M.J. et al. 2018, 'Beneficial effects of folic acid on the kidneys and testes of adult albino rats after exposure to methomyl', Toxicology Research vol. 7, no. 3, pp. 480-491. en_ZA
dc.identifier.issn 2045-4538
dc.identifier.other 10.1039/c7tx00309a
dc.identifier.uri http://hdl.handle.net/2263/65142
dc.language.iso en en_ZA
dc.publisher Royal Society of Chemistry en_ZA
dc.rights © The Royal Society of Chemistry 2018 en_ZA
dc.subject Folic acid en_ZA
dc.subject Kidneys en_ZA
dc.subject Testes en_ZA
dc.subject Methomyl-induced toxicity en_ZA
dc.subject Rats en_ZA
dc.subject Male en_ZA
dc.subject Caspase-3 en_ZA
dc.subject Antioxidant capacity en_ZA
dc.subject Reproductive toxicity en_ZA
dc.subject Apoptosis en_ZA
dc.subject Fertility en_ZA
dc.subject Parameters en_ZA
dc.subject Metabolism en_ZA
dc.subject Antioxidant en_ZA
dc.subject Damage en_ZA
dc.subject Insecticide en_ZA
dc.subject Oxidative stress en_ZA
dc.title Beneficial effects of folic acid on the kidneys and testes of adult albino rats after exposure to methomyl en_ZA
dc.type Postprint Article en_ZA


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