Selected essential oils inhibit key physiological enzymes and possess intracellular and extracellular antimelanogenic properties in vitro

Show simple item record

dc.contributor.author Aumeeruddy-Elalfi, Zaahira
dc.contributor.author Lall, Namrita
dc.contributor.author Fibrich, Bianca
dc.contributor.author Van Staden, Analike Blom
dc.contributor.author Hosenally, Muzzammil
dc.contributor.author Mahomoodally, Mohamad Fawzi
dc.date.accessioned 2018-06-12T08:16:51Z
dc.date.available 2018-06-12T08:16:51Z
dc.date.issued 2018-01
dc.description.abstract Essential oils (EOs) extracted from six medicinal herbs and food plants [Cinnamomum zeylanicum (CZ), Psiadia arguta (PA), Psiadia terebinthina (PT), Citrus grandis (CGp), Citrus hystrix (CH), and Citrus reticulata (CR)] were studied for any inhibitory potential against key physiological enzymes involved in diabetes (a-glucosidase), skin aging (collagenase and elastase), and neurodegenerative disorders (acetylcholinesterase). Kinetic studies of the active EOs on the aforementioned enzymes were determined using LineweavereBurk plots. The intracellular and extracellular antimelanogenic potential of the EOs were evaluated on B16F10 mouse melanocytes. CH and CR were found to significantly inhibit (2.476 ± 0.13 mg/mL and 3.636 ± 0.10 mg/mL, respectively) acetylcholinesterase, compared with galantamine (3.989 ± 0.16 mg/mL). CH inhibited collagenase (50% inhibitory concentration 28.71 ± 0.16 mg/mL) compared with the control (24.45 ± 0.19 mg/mL). The percentage inhibition in the elastase assay of CH was 63.21% compared to the positive control (75.09%). In addition, CH, CR, CGp, CZ, and PT were found to significantly inhibit a-glucosidase (276.70 ± 0.73 mg/mL, 169.90 ± 0.58 mg/mL, 240.60 ± 6.50 mg/mL, 64.52 ± 0.69 mg/mL, and 313.0 ± 5.0 mg/mL, respectively), compared to acarbose (448.80 ± 0.81 mg/mL). Active EOs showed both uncompetitive and competitive types of inhibition. The EOs also inhibited intracellular (50% inhibitory concentration 15.92 ± 1.06 mg/mL, 23.75 ± 4.47 mg/mL, and 28.99 ± 5.70 mg/mL for CH, CR, and CGp, respectively) and extracellular (< 15.625 mg/mL for CH, CR, CGp, and PT) melanin production when tested against B16F10 mouse melanocytes. Results from the present study tend to show that EOs extracted from these medicinal plants can inhibit key enzymes and may be potential candidates for cosmetic and pharmaceutical industries. en_ZA
dc.description.department Plant Production and Soil Science en_ZA
dc.description.librarian am2018 en_ZA
dc.description.uri http://www.jfda-online.com en_ZA
dc.identifier.citation Aumeeruddy-Elalfi, Z., Lall, N., Fibrich, B. 2018, 'Selected essential oils inhibit key physiological enzymes and possess intracellular and extracellular antimelanogenic properties in vitro', Journal of Food and Drug Analysis, vol. 26, no. 1, pp. 232-243. en_ZA
dc.identifier.issn 1021-9498
dc.identifier.other 10.1016/j.jfda.2017.03.002
dc.identifier.uri http://hdl.handle.net/2263/65137
dc.language.iso en en_ZA
dc.publisher Elsevier en_ZA
dc.rights © 2017, Food and Drug Administration, Taiwan. Published by Elsevier Taiwan LLC. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). en_ZA
dc.subject Acetylcholinesterase en_ZA
dc.subject Citrus en_ZA
dc.subject Diabetes mellitus en_ZA
dc.subject Hyperpigmentation en_ZA
dc.subject Oils en_ZA
dc.subject Mauritius en_ZA
dc.subject Activation en_ZA
dc.subject Melanogenesis en_ZA
dc.subject Antioxidant en_ZA
dc.subject Cinnamon en_ZA
dc.subject Melanoma cells en_ZA
dc.subject Medicinal plants en_ZA
dc.subject Oxidative stress en_ZA
dc.subject Alzheimer's disease en_ZA
dc.subject Chemical composition en_ZA
dc.subject Essential oils (EOs) en_ZA
dc.title Selected essential oils inhibit key physiological enzymes and possess intracellular and extracellular antimelanogenic properties in vitro en_ZA
dc.type Article en_ZA


Files in this item

This item appears in the following Collection(s)

Show simple item record