Selected essential oils inhibit key physiological enzymes and possess intracellular and extracellular antimelanogenic properties in vitro

dc.contributor.authorAumeeruddy-Elalfi, Zaahira
dc.contributor.authorLall, Namrita
dc.contributor.authorFibrich, Bianca
dc.contributor.authorVan Staden, Analike Blom
dc.contributor.authorHosenally, Muzzammil
dc.contributor.authorMahomoodally, Mohamad Fawzi
dc.date.accessioned2018-06-12T08:16:51Z
dc.date.available2018-06-12T08:16:51Z
dc.date.issued2018-01
dc.description.abstractEssential oils (EOs) extracted from six medicinal herbs and food plants [Cinnamomum zeylanicum (CZ), Psiadia arguta (PA), Psiadia terebinthina (PT), Citrus grandis (CGp), Citrus hystrix (CH), and Citrus reticulata (CR)] were studied for any inhibitory potential against key physiological enzymes involved in diabetes (a-glucosidase), skin aging (collagenase and elastase), and neurodegenerative disorders (acetylcholinesterase). Kinetic studies of the active EOs on the aforementioned enzymes were determined using LineweavereBurk plots. The intracellular and extracellular antimelanogenic potential of the EOs were evaluated on B16F10 mouse melanocytes. CH and CR were found to significantly inhibit (2.476 ± 0.13 mg/mL and 3.636 ± 0.10 mg/mL, respectively) acetylcholinesterase, compared with galantamine (3.989 ± 0.16 mg/mL). CH inhibited collagenase (50% inhibitory concentration 28.71 ± 0.16 mg/mL) compared with the control (24.45 ± 0.19 mg/mL). The percentage inhibition in the elastase assay of CH was 63.21% compared to the positive control (75.09%). In addition, CH, CR, CGp, CZ, and PT were found to significantly inhibit a-glucosidase (276.70 ± 0.73 mg/mL, 169.90 ± 0.58 mg/mL, 240.60 ± 6.50 mg/mL, 64.52 ± 0.69 mg/mL, and 313.0 ± 5.0 mg/mL, respectively), compared to acarbose (448.80 ± 0.81 mg/mL). Active EOs showed both uncompetitive and competitive types of inhibition. The EOs also inhibited intracellular (50% inhibitory concentration 15.92 ± 1.06 mg/mL, 23.75 ± 4.47 mg/mL, and 28.99 ± 5.70 mg/mL for CH, CR, and CGp, respectively) and extracellular (< 15.625 mg/mL for CH, CR, CGp, and PT) melanin production when tested against B16F10 mouse melanocytes. Results from the present study tend to show that EOs extracted from these medicinal plants can inhibit key enzymes and may be potential candidates for cosmetic and pharmaceutical industries.en_ZA
dc.description.departmentPlant Production and Soil Scienceen_ZA
dc.description.librarianam2018en_ZA
dc.description.urihttp://www.jfda-online.comen_ZA
dc.identifier.citationAumeeruddy-Elalfi, Z., Lall, N., Fibrich, B. 2018, 'Selected essential oils inhibit key physiological enzymes and possess intracellular and extracellular antimelanogenic properties in vitro', Journal of Food and Drug Analysis, vol. 26, no. 1, pp. 232-243.en_ZA
dc.identifier.issn1021-9498
dc.identifier.other10.1016/j.jfda.2017.03.002
dc.identifier.urihttp://hdl.handle.net/2263/65137
dc.language.isoenen_ZA
dc.publisherElsevieren_ZA
dc.rights© 2017, Food and Drug Administration, Taiwan. Published by Elsevier Taiwan LLC. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).en_ZA
dc.subjectAcetylcholinesteraseen_ZA
dc.subjectCitrusen_ZA
dc.subjectDiabetes mellitusen_ZA
dc.subjectHyperpigmentationen_ZA
dc.subjectOilsen_ZA
dc.subjectMauritiusen_ZA
dc.subjectActivationen_ZA
dc.subjectMelanogenesisen_ZA
dc.subjectAntioxidanten_ZA
dc.subjectCinnamonen_ZA
dc.subjectMelanoma cellsen_ZA
dc.subjectMedicinal plantsen_ZA
dc.subjectOxidative stressen_ZA
dc.subjectAlzheimer's diseaseen_ZA
dc.subjectChemical compositionen_ZA
dc.subjectEssential oils (EOs)en_ZA
dc.titleSelected essential oils inhibit key physiological enzymes and possess intracellular and extracellular antimelanogenic properties in vitroen_ZA
dc.typeArticleen_ZA

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