Biological activities of Heteropyxis natalensis against micro-organisms involved in oral infections

Show simple item record Henley-Smith, Cynthia J. Botha, Francien Susanna Hussein, Ahmed A. Nkomo, Mpumelelo Meyer, Debra Lall, Namrita 2018-06-05T07:20:32Z 2018-06-05T07:20:32Z 2018-04-10
dc.description.abstract The use of complementary and alternative medicine from plants in South Africa, as in the rest of the world, continues to increase. Heteropyxis natalensis, known as the Lavender tree, is indigenous to South Africa and is traditionally used for oral care. The ethanolic extract, of the leaves and twigs, of H. natalensis was investigated for antimicrobial activity against selected oral microorganisms. Actinomyces israelii was found to be the most sensitive oral microorganism to the extract, with a minimum inhibitory concentration (MIC) of 0.88 mg/ml and an MIC of 2.6 mg/ml against Streptococcus mutans. Five known compounds were identified from the ethanolic extract of H. natalensis. The compounds were identified as aurentiacin A (1), cardamomin (2), 5-hydroxy-7-methoxy-6-methylflavanone (3), quercetin (4) and 3,5,7-trihydroxyflavan (5). The MICs of the compounds 1 and 4 were found to be 0.06 mg/ml and 1 mg/ml, respectively, against A. israelii. The cytotoxicity, acute and sub-acute toxicity in pre-clinical studies were also determined for H. natalensis. The extract showed moderate cytotoxicity (35.56 0.16 mg/ml) on human monocyte cells. The acute and sub-acute toxicity analysis of H. natalensis indicated the NOEL (noobserved- effect level) at 200 mg/kg. Interleukin-8 (IL-8) is a chemokine that stimulates the recruitment of leukocytes. A significant reduction of IL-8 production by macrophage cells was observed when exposed to the extract of H. natalensis. It is possible that H. natalensis can prevent excessive tissue damage in periodontal diseases through its reduction of inflammation. Enzymatic bioanalysis of lactic and acetic acid production from Streptococcus mutans and Lactobacillus paracasei was done. A reduction in the acid production from each bacterium was observed on exposure to the extract of H. natalensis. Consequently, this increased the pH, which could possibly reduce the demineralization of enamel which may help prevent the formation of dental caries. In addition the extract may be considered for preventing periodontal diseases. en_ZA
dc.description.department Paraclinical Sciences en_ZA
dc.description.department Plant Production and Soil Science en_ZA
dc.description.librarian am2018 en_ZA
dc.description.sponsorship The National Research Foundation (Grant 90355), NRF innovation doctoral scholarship as well as the Gen Foundation Grant (from National Trust, United Kingdom). en_ZA
dc.description.uri en_ZA
dc.identifier.citation Henley-Smith CJ, Botha FS, Hussein AA, Nkomo M, Meyer D and Lall N (2018) Biological Activities of Heteropyxis natalensis Against Micro-Organisms Involved in Oral Infections. Front. Pharmacol. 9:291. DOI: 10.3389/fphar.2018.00291. en_ZA
dc.identifier.issn 1663-9812 (online)
dc.identifier.other 10.3389/fphar.2018.00291
dc.language.iso en en_ZA
dc.publisher Frontiers Media en_ZA
dc.rights © 2018 Henley-Smith, Botha, Hussein, Nkomo, Meyer and Lall. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). en_ZA
dc.subject Heteropyxis natalensis en_ZA
dc.subject Actinomyces israelii en_ZA
dc.subject Antibacterial en_ZA
dc.subject Compound isolation en_ZA
dc.subject Anti-inflammatory en_ZA
dc.subject Monocyte chemoattractant protein-1 en_ZA
dc.subject Interleukin-8 en_ZA
dc.subject Bacteria en_ZA
dc.subject Extracts en_ZA
dc.subject Candida albicans en_ZA
dc.subject Mononuclear cells en_ZA
dc.subject Antifungal activity en_ZA
dc.subject South African plants en_ZA
dc.title Biological activities of Heteropyxis natalensis against micro-organisms involved in oral infections en_ZA
dc.type Article en_ZA

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