Structural characterization and directed evolution of a novel acetyl xylan esterase reveals thermostability determinants of the carbohydrate esterase 7 family

Adesioye, Fiyinfoluwa Adenike; Makhalanyane, Thulani Peter; Vikram, Surendra; Sewell, Bryan T.; Schubert, Wolf-Dieter; Cowan, Don A.

dc.contributor.author	Adesioye, Fiyinfoluwa Adenike
dc.contributor.author	Makhalanyane, Thulani Peter
dc.contributor.author	Vikram, Surendra
dc.contributor.author	Sewell, Bryan T.
dc.contributor.author	Schubert, Wolf-Dieter
dc.contributor.author	Cowan, Don A.
dc.date.accessioned	2018-04-20T10:19:35Z
dc.date.issued	2018-04
dc.description.abstract	Please read abstract in the article.	en_ZA
dc.description.department	Biochemistry	en_ZA
dc.description.department	Genetics	en_ZA
dc.description.embargo	2018-10-01
dc.description.librarian	hj2018	en_ZA
dc.description.sponsorship	The South Africa Bio-catalysis Initiative, Department of Science and Technology, the University of Pretoria Genomics Research Institute (D.A.C. and T.P.M.), the National Research Foundation (W.-D.S. and B.T.S.), the Research Development Program (T.P.M. and S.V.), and the Organization for Women in Science in the Developing World (OWSD) (F.A.A.).	en_ZA
dc.description.uri	http://aem.asm.org	en_ZA
dc.identifier.citation	Adesioye FA, Makhalanyane TP, Vikram S, Sewell BT, Schubert W-D, Cowan DA. 2018. Structural characterization and directed evolution of a novel acetyl xylan esterase reveals thermostability determinants of the carbohydrate esterase 7 family. Appl Environ Microbiol 84:e02695-17. https://doi.org/10.1128/AEM.02695-17.	en_ZA
dc.identifier.issn	0099-2240 (print)
dc.identifier.issn	1098-5336 (online)
dc.identifier.other	10.1128/AEM.02695-17
dc.identifier.uri	http://hdl.handle.net/2263/64669
dc.language.iso	en	en_ZA
dc.publisher	American Society for Microbiology	en_ZA
dc.rights	© 2018, American Society for Microbiology. All Rights Reserved.	en_ZA
dc.subject	X-ray crystallography	en_ZA
dc.subject	Structural characterization	en_ZA
dc.subject	Single-point mutation	en_ZA
dc.subject	Salt concentration	en_ZA
dc.subject	Metagenomics	en_ZA
dc.subject	Directed evolution	en_ZA
dc.subject	Catalytic efficiencies	en_ZA
dc.subject	Biosynthesis	en_ZA
dc.subject	Carbohydrates	en_ZA
dc.subject	Catalyst activity	en_ZA
dc.subject	Cloning	en_ZA
dc.subject	Crystal structure	en_ZA
dc.subject	Efficiency	en_ZA
dc.subject	Enzymes	en_ZA
dc.subject	Esters	en_ZA
dc.subject	Genes	en_ZA
dc.subject	Sodium compounds	en_ZA
dc.subject	Thermodynamic stability	en_ZA
dc.subject	Acetyl xylan esterase (AcXE)	en_ZA
dc.subject	Carbohydrate esterase 7 (CE7)	en_ZA
dc.subject	Sequence-based metagenomics	en_ZA
dc.subject	Thermal stability	en_ZA
dc.title	Structural characterization and directed evolution of a novel acetyl xylan esterase reveals thermostability determinants of the carbohydrate esterase 7 family	en_ZA
dc.type	Postprint Article	en_ZA