Geigerin-induced cytotoxicity in a murine myoblast cell line (C2C12)

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dc.contributor.author Botha, C.J. (Christoffel Jacobus)
dc.contributor.author Clift, Sarah J.
dc.contributor.author Ferreira, Gezina Catharina Helena
dc.contributor.author Masango, Mxolisi Goodwill
dc.date.accessioned 2018-03-12T08:57:40Z
dc.date.available 2018-03-12T08:57:40Z
dc.date.issued 2017-10-31
dc.description.abstract Geigeria poisoning in sheep, locally known as ‘vermeersiekte’, is an economically important plant poisoning in southern Africa. The toxic principles contained by the toxic plants are believed to be several sesquiterpene lactones, such as geigerin, vermeeric acid and vermeerin, which cause striated muscle lesions in small stock. Because of ethical issues surrounding the use of live animals in toxicity studies, there is currently a dire need to establish an in vitro model that can be used to replace traditional animal experimentation. The objective of this study was to determine the cytotoxicity of geigerin in a murine myoblast cell line (C2C12) using methyl-thiazol-tetrazolium (MTT) and lactate dehydrogenase (LDH) assays, annexin V and propidium iodide (PI) flow cytometry and transmission electron microscopy (TEM). Mouse myoblasts were exposed to 2.0 mM, 2.5 mM and 5.0 mM geigerin for 24, 48 and 72 h. A concentration-dependent cytotoxic response was observed. Apoptosis was detected by means of annexin V flow cytometry during the first 24 h and apoptotic bodies were also visible on TEM. According to the LDH and PI flow cytometry results, myoblast cell membranes were not injured. We concluded that the murine myoblast cell line (C2C12) is a suitable model for future studies planned to evaluate the cytotoxicity of other and combinations of sesquiterpene lactones, with and without metabolic activation, implicated in ‘vermeersiekte’ and to elucidate the subcellular effects of these myotoxins on cultured myoblasts. en_ZA
dc.description.department Paraclinical Sciences en_ZA
dc.description.librarian am2018 en_ZA
dc.description.sponsorship The National Research Foundation (NRF) of South Africa en_ZA
dc.description.uri http://www.ojvr.org en_ZA
dc.identifier.citation Botha, C.J., Clift, S.J., Ferreira, G.C.H. & Masango, M.G., 2017, ‘Geigerin-induced cytotoxicity in a murine myoblast cell line (C2C12)’, Onderstepoort Journal of Veterinary Research 84(1), a1465. https://DOI.org/ 10.4102/ojvr.v84i1.1465. en_ZA
dc.identifier.issn 0030-2465 (print)
dc.identifier.issn 2219-0635 (online)
dc.identifier.other 10.4102/ojvr.v84i1.1465
dc.identifier.uri http://hdl.handle.net/2263/64202
dc.language.iso en en_ZA
dc.publisher AOSIS OpenJournals en_ZA
dc.rights © 2017. The Authors. Licensee: AOSIS. This work is licensed under the Creative Commons Attribution License. en_ZA
dc.subject Sheep en_ZA
dc.subject Methyl-thiazol-tetrazolium (MTT) en_ZA
dc.subject Lactate dehydrogenase (LDH) en_ZA
dc.subject Geigeria poisoning en_ZA
dc.subject Vermeersiekte en_ZA
dc.subject Plant poisoning en_ZA
dc.subject Transmission electron microscopy (TEM) en_ZA
dc.subject Annexin V en_ZA
dc.subject Propidium iodide (PI) en_ZA
dc.subject Sesquiterpene lactones en_ZA
dc.subject Oxidative phosphorylation en_ZA
dc.subject Induced apoptosis en_ZA
dc.subject South Africa (SA) en_ZA
dc.subject Inhibition en_ZA
dc.subject Generation en_ZA
dc.subject Necrosis en_ZA
dc.title Geigerin-induced cytotoxicity in a murine myoblast cell line (C2C12) en_ZA
dc.type Article en_ZA


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