Geigerin-induced cytotoxicity in a murine myoblast cell line (C2C12)

dc.contributor.authorBotha, C.J. (Christoffel Jacobus)
dc.contributor.authorClift, Sarah Jane
dc.contributor.authorFerreira, Gezina Catharina Helena
dc.contributor.authorMasango, Mxolisi Goodwill
dc.contributor.emailchristo.botha@up.ac.zaen_ZA
dc.date.accessioned2018-03-12T08:57:40Z
dc.date.available2018-03-12T08:57:40Z
dc.date.issued2017-10-31
dc.description.abstractGeigeria poisoning in sheep, locally known as ‘vermeersiekte’, is an economically important plant poisoning in southern Africa. The toxic principles contained by the toxic plants are believed to be several sesquiterpene lactones, such as geigerin, vermeeric acid and vermeerin, which cause striated muscle lesions in small stock. Because of ethical issues surrounding the use of live animals in toxicity studies, there is currently a dire need to establish an in vitro model that can be used to replace traditional animal experimentation. The objective of this study was to determine the cytotoxicity of geigerin in a murine myoblast cell line (C2C12) using methyl-thiazol-tetrazolium (MTT) and lactate dehydrogenase (LDH) assays, annexin V and propidium iodide (PI) flow cytometry and transmission electron microscopy (TEM). Mouse myoblasts were exposed to 2.0 mM, 2.5 mM and 5.0 mM geigerin for 24, 48 and 72 h. A concentration-dependent cytotoxic response was observed. Apoptosis was detected by means of annexin V flow cytometry during the first 24 h and apoptotic bodies were also visible on TEM. According to the LDH and PI flow cytometry results, myoblast cell membranes were not injured. We concluded that the murine myoblast cell line (C2C12) is a suitable model for future studies planned to evaluate the cytotoxicity of other and combinations of sesquiterpene lactones, with and without metabolic activation, implicated in ‘vermeersiekte’ and to elucidate the subcellular effects of these myotoxins on cultured myoblasts.en_ZA
dc.description.departmentParaclinical Sciencesen_ZA
dc.description.librarianam2018en_ZA
dc.description.sponsorshipThe National Research Foundation (NRF) of South Africaen_ZA
dc.description.urihttp://www.ojvr.orgen_ZA
dc.identifier.citationBotha, C.J., Clift, S.J., Ferreira, G.C.H. & Masango, M.G., 2017, ‘Geigerin-induced cytotoxicity in a murine myoblast cell line (C2C12)’, Onderstepoort Journal of Veterinary Research 84(1), a1465. https://DOI.org/ 10.4102/ojvr.v84i1.1465.en_ZA
dc.identifier.issn0030-2465 (print)
dc.identifier.issn2219-0635 (online)
dc.identifier.other10.4102/ojvr.v84i1.1465
dc.identifier.urihttp://hdl.handle.net/2263/64202
dc.language.isoenen_ZA
dc.publisherAOSIS OpenJournalsen_ZA
dc.rights© 2017. The Authors. Licensee: AOSIS. This work is licensed under the Creative Commons Attribution License.en_ZA
dc.subjectSheepen_ZA
dc.subjectMethyl-thiazol-tetrazolium (MTT)en_ZA
dc.subjectLactate dehydrogenase (LDH)en_ZA
dc.subjectGeigeria poisoningen_ZA
dc.subjectVermeersiekteen_ZA
dc.subjectPlant poisoningen_ZA
dc.subjectTransmission electron microscopy (TEM)en_ZA
dc.subjectAnnexin Ven_ZA
dc.subjectPropidium iodide (PI)en_ZA
dc.subjectSesquiterpene lactonesen_ZA
dc.subjectOxidative phosphorylationen_ZA
dc.subjectInduced apoptosisen_ZA
dc.subjectSouth Africa (SA)en_ZA
dc.subjectInhibitionen_ZA
dc.subjectGenerationen_ZA
dc.subjectNecrosisen_ZA
dc.titleGeigerin-induced cytotoxicity in a murine myoblast cell line (C2C12)en_ZA
dc.typeArticleen_ZA

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