First isolation of glutinol and a bioactive fraction with good anti-inflammatory activity from n-hexane fraction of Peltophorum africanum leaf

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dc.contributor.author Adebayo, S.A.
dc.contributor.author Shai, Leshweni J.
dc.contributor.author Eloff, Jacobus Nicolaas
dc.date.accessioned 2017-02-13T06:39:50Z
dc.date.available 2017-02-13T06:39:50Z
dc.date.issued 2017-01
dc.description.abstract OBJECTIVE : To investigate the anti-inflammatory activity of different fractions and glutinol (isolated compound), using nitric oxide synthase and cyclooxygenase (COX) inhibition as an indication of anti-inflammatory activity. METHODS : Anti-inflammatory activity was evaluated using an in vitro assay determining the inhibition of the activity of pro-inflammatory enzyme model. Cyclooxygenases and inducible nitric oxide synthase are crucial enzymes involved in the pathogenesis of many chronic inflammatory conditions. RESULTS : Sub-fraction F3.3 that was derived from n-hexane fraction of PA leaves significantly inhibited (P = 0.01) the catalytic activity of COX-2 (IC50 = 0.67 mg/mL) better than isolated compound, glutinol (IC50 = 1.22 mg/mL), compound 2 (CP2) (IC50 = 1.71 mg/mL) and sub-fraction F3.3.0 (IC50 = 1.30 mg/mL). A similar trend was observed in investigation of the inhibition of nitric oxide synthesis in RAW 264.7 cells by F3.3, glutinol, CP2 and F3.3.0. Inducible COX-2 and inducible nitric oxide synthase are among potent signalling enzymes that exacerbate inflammation. CONCLUSIONS : Bioactive sub-fractions (F3.3 and F3.3.0) derived from the n-hexane fraction of PA had good anti-inflammatory activity, and the isolated compound, and glutinol may be useful as a template for the development of new anti-inflammatory drugs. en_ZA
dc.description.department Paraclinical Sciences en_ZA
dc.description.librarian am2017 en_ZA
dc.description.sponsorship The National Research Foundation of South Africa (No. Eloff IPPR 953991) en_ZA
dc.description.uri http://www.elsevier.com/locate/apjtm en_ZA
dc.identifier.citation Adebayo, SA, Shai, LJ & Eloff, JN 2017, 'First isolation of glutinol and a bioactive fraction with good anti-inflammatory activity from n-hexane fraction of Peltophorum africanum leaf', Asian Pacific Journal of Tropical Medicine, vol. 10, no. 1, pp. 42-46. en_ZA
dc.identifier.issn 1995-7645
dc.identifier.other 10.1016/j.apjtm.2016.12.004
dc.identifier.uri http://hdl.handle.net/2263/59000
dc.language.iso en en_ZA
dc.publisher Elsevier en_ZA
dc.rights © 2017 Hainan Medical University. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license. en_ZA
dc.subject Anti-inflammatory en_ZA
dc.subject Cyclooxygenases 2 (COX-2) en_ZA
dc.subject Nitric oxide synthase (iNOS) en_ZA
dc.subject Cyclooxygenases 1 (COX-1) en_ZA
dc.subject Nitric oxide (NO) en_ZA
dc.subject Peltophorum africanum (PA) en_ZA
dc.title First isolation of glutinol and a bioactive fraction with good anti-inflammatory activity from n-hexane fraction of Peltophorum africanum leaf en_ZA
dc.type Article en_ZA


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