dc.contributor.author |
Rahimlou, S.
|
|
dc.contributor.author |
Babaeizad, V.
|
|
dc.contributor.author |
Sayari, Mohammad
|
|
dc.date.accessioned |
2016-09-12T07:08:06Z |
|
dc.date.available |
2016-09-12T07:08:06Z |
|
dc.date.issued |
2014 |
|
dc.description.abstract |
Pomegranate (Punica granatum) is one of the most important commercial fruit crop in eastern Mazandaran (Iran, 35°47’N, 50°34’E). During spring 2013, distinct dark brown spots were observed on pomegranate fruits, from which a fungus was isolated on standard potato dextrose agar (PDA) amended with streptomycin (0.05% w/v). The mycelium was white- grey turning olive green over time, and produced oval to cylindrical, hyaline, unicellular, aseptate conidia measuring 5-13×1.5-4 μm. Based on these morphological characters the mycete was tentatively identified as Colletotrichum gloeosporioides. The fungal internal transcribed spacer (ITS) region of r-DNA was then amplified using the primers ITS5/ITS4 sequenced locally and deposited under GeneBank accession No. KJ769129. A sequence similarity search performed using BLAST (Altschul et al. 1990) algorithm available via GenBank confirmed the identification as C. gloeosporioides. Pathogenicity tests were carried out by placing agar-discs from a six-day-old culture of the fungus onto five artificially injured pomegranate fruits, which were placed inside sterile plastic bags. Controls consisted of non- inoculated fruits. Symptoms were reproduced after six days only on inoculated fruits and the pathogen was subsequently re-isolated, fulfilling Koch’s postulates. To our knowledge, this is the first report of. C. gloeosporioides in pomegranate fruits in Iran. |
en_ZA |
dc.description.department |
Microbiology and Plant Pathology |
en_ZA |
dc.description.librarian |
am2016 |
en_ZA |
dc.description.uri |
http://www.sipav.org/main/jpp/index.php/jpp |
en_ZA |
dc.identifier.citation |
Rahimlou, S, Babaeizad, V & Sayari, M 2014, 'First report of fruit spot of pomegranate caused by Colletotrichum Gloeosporioides in Iran', Journal of Plant Pathology, vol. 96, no. 3, pp. 605. |
en_ZA |
dc.identifier.issn |
1125-4653 (print) |
|
dc.identifier.issn |
2239-7264 (online) |
|
dc.identifier.uri |
http://hdl.handle.net/2263/56699 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
Edizioni ETS |
en_ZA |
dc.rights |
Italian Phytopathological Society |
en_ZA |
dc.subject |
Pomegranate fruit |
en_ZA |
dc.subject |
Pathogenicity tests |
en_ZA |
dc.subject |
Colletotrichum gloeosporioides |
en_ZA |
dc.subject |
Internal transcribed spacer (ITS) |
en_ZA |
dc.subject |
Potato dextrose agar (PDA) |
en_ZA |
dc.title |
First report of fruit spot of pomegranate caused by Colletotrichum Gloeosporioides in Iran |
en_ZA |
dc.type |
Article |
en_ZA |