Comprehensive genome-wide analysis of the Aux/IAA gene family in Eucalyptus : evidence for the role of EgrIAA4 in wood formation

Show simple item record Yu, Hong Soler, Marçal Clemente, Hélène San Mila, Isabelle Paiva, Jorge A.P. Myburg, Alexander Andrew Bouzayen, Mondher Grima-Pettenati, Jacqueline Cassan-Wang, Hua 2016-08-31T11:42:27Z 2016-08-31T11:42:27Z 2015-04
dc.description.abstract Auxin plays a pivotal role in various plant growth and development processes, including vascular differentiation. The modulation of auxin responsiveness through the auxin perception and signaling machinery is believed to be a major regulatory mechanism controlling cambium activity and wood formation. To gain more insights into the roles of key Aux/IAA gene regulators of the auxin response in these processes, we identified and characterized members of the Aux/IAA family in the genome of Eucalyptus grandis, a tree of worldwide economic importance. We found that the gene family in Eucalyptus is slightly smaller than that in Populus and Arabidopsis, but all phylogenetic groups are represented. High-throughput expression profiling of different organs and tissues highlighted several Aux/IAA genes expressed in vascular cambium and/or developing xylem, some showing differential expression in response to developmental (juvenile vs. mature) and/or to environmental (tension stress) cues. Based on the expression profiles, we selected a promising candidate gene, EgrIAA4, for functional characterization. We showed that EgrIAA4 protein is localized in the nucleus and functions as an auxin-responsive repressor. Overexpressing a stabilized version of EgrIAA4 in Arabidopsis dramatically impeded plant growth and fertility and induced auxin-insensitive phenotypes such as inhibition of primary root elongation, lateral root emergence and agravitropism. Interestingly, the lignified secondary walls of the interfascicular fibers appeared very late, whereas those of the xylary fibers were virtually undetectable, suggesting that EgrIAA4 may play crucial roles in fiber development and secondary cell wall deposition. en_ZA
dc.description.department Genetics en_ZA
dc.description.librarian hb2016 en_ZA
dc.description.sponsorship This work was supported by the Centre National pour la Recherche Scientifique (CNRS); the University Paul Sabatier Toulouse III (UPS); the Agence Nationale de la Recherche (ANR); the French Laboratory of Excellence *project ‘TULIP’ (ANR-10-LABX-41; ANR-11-IDEX-0002-02)]; the Plant KBBE TreeForJoules [project ANR-2010-KBBE-007-01 (FR) and PKBBE/ AGR_GPL/0001/2010 (FCT, PT) and the project microEGo (PTDC/AGRGPL/ 098179/2008; FCT, PT); the China Scholarship Council [a PhD grant to H.Y.]; the Departament d’Universitats, Recerca i Societat de la Informació de la Generalitat de Catalunya [a post-doctoral fellowship ‘Beatriu de Pinós’ to M.S.+; the Fundação para a Ciência e a Tecnologia (FCT) [a research contract from the Ciência 2008 program and a postdoctoral fellowship SFRH/BPD/92207/2013 to J.A.P.P.]. en_ZA
dc.description.uri en_ZA
dc.identifier.citation Yu, H, Soler, M, Clemente, HS, Mila, I, Paiva, JAP, Myburg, AA, Bouzayen, M, Grima-Pettenati, J & Cassan-Wang, H 2015, 'Comprehensive genome-wide analysis of the Aux/IAA gene family in Eucalyptus : evidence for the role of EgrIAA4 in wood formation', Plant and Cell Physiology, vol. 56, no. 4, pp. 700-714. en_ZA
dc.identifier.issn 0032-0781 (print)
dc.identifier.issn 1471-9053 (online)
dc.identifier.other 10.1093/pcp/pcu215
dc.language.iso en en_ZA
dc.publisher Oxford University Press en_ZA
dc.rights © The Author 2015. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Plant and Cell Physiology following peer review. The definitive publisher-authenticated version is : Title, Plant and Cell Physiology, vol. 56, no. 4, pp. 700-714, 2015. doi : 10.1093/pcp/pcu215, is available online at : en_ZA
dc.subject Aux/IAA en_ZA
dc.subject Auxin en_ZA
dc.subject Eucalyptus en_ZA
dc.subject Gene expression en_ZA
dc.subject Secondary cell wall (SCW) en_ZA
dc.subject Wood formation en_ZA
dc.title Comprehensive genome-wide analysis of the Aux/IAA gene family in Eucalyptus : evidence for the role of EgrIAA4 in wood formation en_ZA
dc.type Postprint Article en_ZA

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