dc.contributor.author |
Reeve, Richard
|
|
dc.contributor.author |
Borley, Daryl W.
|
|
dc.contributor.author |
Maree, Francois Frederick
|
|
dc.contributor.author |
Upadhyaya, Sasmita
|
|
dc.contributor.author |
Lukhwareni, Azwidowi
|
|
dc.contributor.author |
Esterhuysen, Jan J.
|
|
dc.contributor.author |
Harvey, William T.
|
|
dc.contributor.author |
Blignaut, Belinda
|
|
dc.contributor.author |
Fry, Elizabeth E.
|
|
dc.contributor.author |
Parida, Satya
|
|
dc.contributor.author |
Paton, David J.
|
|
dc.contributor.author |
Mahapatra, Mana
|
|
dc.date.accessioned |
2016-08-24T06:54:11Z |
|
dc.date.available |
2016-08-24T06:54:11Z |
|
dc.date.issued |
2016-07-22 |
|
dc.description |
S1 Data. VNT serological results for serotype O viruses and antisera. |
en_ZA |
dc.description |
S2 Data. LPBE serological results for serotype O viruses and antisera. |
en_ZA |
dc.description |
S3 Data. VNT serological results for serotype SAT1 viruses and antisera. |
en_ZA |
dc.description |
S1 Table. Foot-and-mouth disease virus details with accession numbers. |
en_ZA |
dc.description |
S2 Table. Pan-serotypic reference alignment of FMDV. The dataset shows the aligned VP2,
VP3 and VP1 proteins of example SAT1 and O isolates used in the study alongside representative
isolates from the other five serotypes. The four contiguous surface-exposed structural
motifs confirmed as containing antigenic sites on at least four serotypes are highlighted in red–locations are approximate due to structural differences between the serotypes. The RGD cell
surface receptor-binding motif, in the centre of the third site, is highlighted in blue. |
en_ZA |
dc.description |
S3 Table. Residues identified as part of epitopes on structural proteins across the six tested
serotypes of FMDV, along with corresponding positions on all serotypes. |
en_ZA |
dc.description |
S4 Table. SAT1 mar-mutants. |
en_ZA |
dc.description.abstract |
Quantifying and predicting the antigenic characteristics of a virus is something of a holy
grail for infectious disease research because of its central importance to the emergence of
new strains, the severity of outbreaks, and vaccine selection. However, these characteristics
are defined by a complex interplay of viral and host factors so that phylogenetic measures
of viral similarity are often poorly correlated to antigenic relationships. Here, we
generate antigenic phylogenies that track the phenotypic evolution of two serotypes of footand-
mouth disease virus by combining host serology and viral sequence data to identify
sites that are critical to their antigenic evolution. For serotype SAT1, we validate our antigenic
phylogeny against monoclonal antibody escape mutants, which match all of the predicted
antigenic sites. For serotype O, we validate it against known sites where available,
and otherwise directly evaluate the impact on antigenic phenotype of substitutions in predicted
sites using reverse genetics and serology. We also highlight a critical and poorly
understood problem for vaccine selection by revealing qualitative differences between
assays that are often used interchangeably to determine antigenic match between field
viruses and vaccine strains. Our approach provides a tool to identify naturally occurring antigenic
substitutions, allowing us to track the genetic diversification and associated antigenic
evolution of the virus. Despite the hugely important role vaccines have played in enhancing
human and animal health, vaccinology remains a conspicuously empirical science. This
study advances the field by providing guidance for tuning vaccine strains via site-directed
mutagenesis through this high-resolution tracking of antigenic evolution of the virus
between rare major shifts in phenotype. |
en_ZA |
dc.description.department |
Microbiology and Plant Pathology |
en_ZA |
dc.description.department |
Production Animal Studies |
en_ZA |
dc.description.librarian |
am2016 |
en_ZA |
dc.description.sponsorship |
The authors acknowledge the
Biotechnology and Biological Sciences Research
Council (BBSRC) Institute Strategic Programme on
Livestock Viral Diseases at The Pirbright Institute
(BB/J004375/1) [RR SP DJP MM] and BBSRC BB/
G529532/1 [DWB MM], BB/F009186/1 [MM] and
BBSRC BB/L004828 [RR] and BBSRC / Department
for International Development / Scottish Government
grants BB/H009302/1 [RR] and BB/H009175/1 [SP
FFM RR] (http://www.bbsrc.ac.uk), and Department
for Environment, Food and Rural Affairs grant
SE2937 (http://www.gov.uk/defra) [MM]. The Food
and Agriculture Organisation financially supported the
research to determine one-way relationship and
antigenic relatedness of SAT1 viruses under grants
OSRO/RAF/721/EC and MTF/INT/003/EEC (http://
www.fao.org) [FFM AL JJE BB], and RMRSA (http://
www.rmrdsa.co.za) (Improving detection and
characterisation methods for FMDV and ASFV for
cattle and pigs in the SADC region) [BB]. Structural
studies supported by the UK Medical Research
Council grant MR/N00065X/1 [EEF] (http://www.mrc.
ac.uk). The work of the Wellcome Trust Centre in
Oxford is supported by the Wellcome Trust core
award 090532/Z/07/Z [EEF] (http://www.wellcome.ac.
uk). |
en_ZA |
dc.description.uri |
http://www.plosone.org |
en_ZA |
dc.identifier.citation |
Reeve R, Borley DW, Maree FF,
Upadhyaya S, Lukhwareni A, Esterhuysen JJ, et al.
(2016) Tracking the Antigenic Evolution of Foot-and-
Mouth Disease Virus. PLoS ONE 11(7): e0159360.
DOI: 10.1371/journal.pone.0159360. |
en_ZA |
dc.identifier.issn |
1932-6203 |
|
dc.identifier.other |
10.1371/journal.pone.0159360 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/56465 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
Public Library of Science |
en_ZA |
dc.rights |
© 2016 Reeve et al. This is an open
access article distributed under the terms of the
Creative Commons Attribution License. |
en_ZA |
dc.subject |
Serotype SAT1 |
en_ZA |
dc.subject |
Serotype O |
en_ZA |
dc.subject |
Infectious disease |
en_ZA |
dc.subject |
Virus |
en_ZA |
dc.subject.other |
Veterinary science articles SDG-01 |
en_ZA |
dc.subject.other |
Veterinary science articles SDG-02 |
en_ZA |
dc.subject.other |
SDG-01: No poverty |
|
dc.subject.other |
SDG-02: Zero hunger |
|
dc.title |
Tracking the antigenic evolution of foot-and-mouth disease virus |
en_ZA |
dc.type |
Article |
en_ZA |