Recovery of nematodes from ruminants by migration from gastro-intestinal ingesta and mucosa gelled in agar : preliminary report
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Date
Authors
Van Wyk, Jan Aucamp
Gerber, H.M.
Journal Title
Journal ISSN
Volume Title
Publisher
The Government Printer, Pretoria
Abstract
When gastro-intestinal ingesta and mucosa containing larvae or adult Haemonchus contortus, Ostertagia circumcincta, Trichostrongylus colubriformis, Strongyloides papillosus, Nematodirus spathiger, Gaigeria pachyscelis, Oesophagostomum columbianum or Chabertia ovina were suspended in 0,75-1,00% agar gel and incubated in physiological saline, a mean of 93,6% of the helminths migrated from the agar-ingesta or agar-mucosa into the physiological saline. The lowest suitable concentration of agar was 0,85-0,90% for one batch of agar and 0,65% for another. Although most of the worms had migrated from the agar gel after 3-4 h of incubation, migration usually continued for longer than 7 h. While low concentrations of formalin partially inhibited migration from the gel, slightly more worms were recovered from agar containing 1 % bile than from agar alone.
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Keywords
Veterinary Medicine
Sustainable Development Goals
Citation
Van Wyk, JA & Gerber, HM 1978, 'Recovery of nematodes from ruminants by migration from gastrointestinal ingesta and mucosa gelled in agar : preliminary report’, Onderstepoort Journal of Veterinary Research, vol. 45, no. 1, pp. 29-37