Isolation and characterization of antibodies to Clostridium perfringens epsilon toxin from hyperimmune horse serum
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Date
Authors
Worthington, R.W.
Mulders, M.S.G.
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Published by The Government Printer, Pretoria
Abstract
Antibodies against epsilon toxin were isolated from hyperimmune horse serum by affinity chromatography. Purified epsilon prototoxin covalently bound to Affigel 202 was used as immunosorbent, and antibodies were eluted with 6,0 M guanidine chloride. In a single run 80 mg of antibody could be recovered from a 20 mℓ column of immunosorbent. The antibody was shown to belong to the IgG(T) class of immunoglobulins.
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Keywords
Veterinary medicine
Sustainable Development Goals
Citation
Worthington, RW & Mulders, MSG 1979, 'Isolation and characterization of antibodies to Clostridium perfringens epsilon toxin from hyperimmune horse serum’, Onderstepoort Journal of Veterinary Research, vol. 46, no. 3, pp. 121-124.