Isolation and characterization of antibodies to Clostridium perfringens epsilon toxin from hyperimmune horse serum

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dc.contributor.author Worthington, R.W.
dc.contributor.author Mulders, M.S.G.
dc.contributor.editor Bigalke, R.D.
dc.contributor.editor Morren, A.J.
dc.contributor.editor Verster, Anna J.M.
dc.contributor.editor Verwoerd, Daniel Wynand
dc.contributor.editor Walker, Jane B.
dc.contributor.editor Cameron, Colin McKenzie
dc.contributor.editor Gilchrist, Frances M.C.
dc.contributor.other Steyn, P.J.J.
dc.date.accessioned 2016-07-06T12:27:31Z
dc.date.available 2016-07-06T12:27:31Z
dc.date.created 2016
dc.date.issued 1979
dc.description This article has been scanned in colour with a HP Scanjet 5590; 300dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-Format. en_ZA
dc.description.abstract Antibodies against epsilon toxin were isolated from hyperimmune horse serum by affinity chromatography. Purified epsilon prototoxin covalently bound to Affigel 202 was used as immunosorbent, and antibodies were eluted with 6,0 M guanidine chloride. In a single run 80 mg of antibody could be recovered from a 20 mℓ column of immunosorbent. The antibody was shown to belong to the IgG(T) class of immunoglobulins. en_ZA
dc.description.sponsorship University of Pretoria Research Fund en_ZA
dc.identifier.citation Worthington, RW & Mulders, MSG 1979, 'Isolation and characterization of antibodies to Clostridium perfringens epsilon toxin from hyperimmune horse serum’, Onderstepoort Journal of Veterinary Research, vol. 46, no. 3, pp. 121-124. en_ZA
dc.identifier.issn 0330-2465
dc.identifier.uri http://hdl.handle.net/2263/53829
dc.language.iso en en_ZA
dc.publisher Published by The Government Printer, Pretoria en_ZA
dc.rights ©1979 ARC- Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original) ©2016 University of Pretoria Department of Library Services(digital) en_ZA
dc.subject Veterinary medicine en_ZA
dc.subject.lcsh Veterinary medicine -- South Africa
dc.title Isolation and characterization of antibodies to Clostridium perfringens epsilon toxin from hyperimmune horse serum en_ZA
dc.type Article en_ZA


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