Isolation and characterization of antibodies to Clostridium perfringens epsilon toxin from hyperimmune horse serum

dc.contributor.authorWorthington, R.W.
dc.contributor.authorMulders, M.S.G.
dc.contributor.editorBigalke, R.D.
dc.contributor.editorMorren, A.J.
dc.contributor.editorVerster, Anna J.M.
dc.contributor.editorVerwoerd, Daniel Wynand
dc.contributor.editorWalker, Jane B.
dc.contributor.editorCameron, Colin McKenzie
dc.contributor.editorGilchrist, Frances M.C.
dc.contributor.otherSteyn, P.J.J.
dc.date.accessioned2016-07-06T12:27:31Z
dc.date.available2016-07-06T12:27:31Z
dc.date.created2016
dc.date.issued1979
dc.descriptionThis article has been scanned in colour with a HP Scanjet 5590; 300dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-Format.en_ZA
dc.description.abstractAntibodies against epsilon toxin were isolated from hyperimmune horse serum by affinity chromatography. Purified epsilon prototoxin covalently bound to Affigel 202 was used as immunosorbent, and antibodies were eluted with 6,0 M guanidine chloride. In a single run 80 mg of antibody could be recovered from a 20 mℓ column of immunosorbent. The antibody was shown to belong to the IgG(T) class of immunoglobulins.en_ZA
dc.description.sponsorshipUniversity of Pretoria Research Funden_ZA
dc.identifier.citationWorthington, RW & Mulders, MSG 1979, 'Isolation and characterization of antibodies to Clostridium perfringens epsilon toxin from hyperimmune horse serum’, Onderstepoort Journal of Veterinary Research, vol. 46, no. 3, pp. 121-124.en_ZA
dc.identifier.issn0330-2465
dc.identifier.urihttp://hdl.handle.net/2263/53829
dc.language.isoenen_ZA
dc.publisherPublished by The Government Printer, Pretoriaen_ZA
dc.rights©1979 ARC- Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original) ©2016 University of Pretoria Department of Library Services(digital)en_ZA
dc.subjectVeterinary medicineen_ZA
dc.subject.lcshVeterinary medicine -- South Africa
dc.titleIsolation and characterization of antibodies to Clostridium perfringens epsilon toxin from hyperimmune horse serumen_ZA
dc.typeArticleen_ZA

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