dc.contributor.author |
Bremer, C.W.
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|
dc.contributor.author |
Barber, T.L.
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|
dc.contributor.editor |
Bigalke, R.D. |
|
dc.contributor.editor |
Morren, A.J. |
|
dc.contributor.editor |
Verster, Anna J.M. |
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dc.contributor.editor |
Verwoerd, Daniel Wynand |
|
dc.contributor.editor |
Walker, Jane B. |
|
dc.contributor.editor |
Cameron, Colin McKenzie |
|
dc.contributor.editor |
Gilchrist, Frances M.C. |
|
dc.contributor.other |
Steyn, P.J.J. |
|
dc.contributor.upauthor |
Huismans, H. (Henk), 1942-
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dc.date.accessioned |
2016-07-06T12:00:54Z |
|
dc.date.available |
2016-07-06T12:00:54Z |
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dc.date.created |
2016 |
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dc.date.issued |
1979 |
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dc.description |
This article has been scanned in colour with a HP Scanjet 5590; 300dpi.
Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-Format. |
en_ZA |
dc.description.abstract |
Purified epizootic haemorrhagic disease virus (EHDV) was shown to contain 10 double-stranded RNA segments and a double-layered protein capsid with 4 major and 4 minor polypeptides. The virus differed from bluetongue virus (BTV), the orbivirus prototype, in that EHDV had an additional minor polypeptide component. This component, together with the major polypeptides P2 and P5, formed the outer capsid layer of the virus. The extra polypeptide apparently stabilizes this layer since, unlike BTV, EHDV was quite stable on CsC1 gradients at both pH 7,0 and 8,0. EHD virions were found to have a density of 1,36 g/mℓ, while particles without the outer capsid layer were isolated and had a density of 1,40 g/mℓ. Two non-capsid polypeptides, P5A and P6A, were identified in addition to the 8 capsid polypeptides. Polypeptide P5A was synthesized in excess of all the others. There was little homology between the nucleic acids of EHDV and BTV with only 5-10% cross-hybridization. No hybrid double-stranded RNA segments were identified. We found by cross-immune precipitation that the major core polypeptides of the 2 viruses (P7 and P3) have common antigenic determinants. |
en_ZA |
dc.identifier.citation |
Huismans, H, Bremer, CW & Barber, TL 1979, 'The nucleic acid and proteins of epizootic haemorrhagic disease virus’, Onderstepoort Journal of Veterinary Research, vol. 46, no. 2, pp. 95-104. |
en_ZA |
dc.identifier.issn |
0330-2465 |
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dc.identifier.uri |
http://hdl.handle.net/2263/53779 |
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dc.language.iso |
en |
en_ZA |
dc.publisher |
Published by The Government Printer, Pretoria |
en_ZA |
dc.rights |
©1979 ARC - Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). ©2016 University of Pretoria Department of Library Services (digital). |
en_ZA |
dc.subject |
Veterinary medicine |
en_ZA |
dc.subject.lcsh |
Veterinary medicine -- South Africa |
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dc.title |
The nucleic acid and proteins of epizootic haemorrhagic disease virus |
en_ZA |
dc.type |
Article |
en_ZA |