The partial purification of Clostridium perfringens Beta toxin

Show simple item record Mulders, M.S.G. Worthington, R.W.
dc.contributor.editor Bigalke, R.D.
dc.contributor.editor Cameron, Colin McKenzie
dc.contributor.editor Verster, Anna J,M.
dc.contributor.editor Walker, Jane B.
dc.contributor.other De Kock, V.E. 2016-07-06T11:10:13Z 2016-07-06T11:10:13Z 2016 1975
dc.description The articles have been scanned in colour with a HP Scanjet 5590; 300dpi. Adobe Acroabt XI was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. en_ZA
dc.description.abstract An attempt was made to purify Clostridium perfringens Beta toxin. Crude toxin prepared by ammonium sulphate precipitation of culture supernatants was purified by chromatography on Sephadex G50, Sephadex G100 and DEAE cellulose. This material, although highly purified, was not homogeneous on polyacrylamide gel electrophoresis. It had a toxicity of 800 000 mouse MLDs/mg N, a typical protein absorption spectrum in the UV region, an iso-electric point of 5, 6 and the main component had a molecular mass of 42 000 ± 2 000 (estimated by electrophoresis in sodium dodecyl sulphate containing polyacrylamide gels) en_ZA
dc.identifier.citation Worthington, RW & Mulders, MSG 1975, 'The partial purification of Clostridium perfringens Beta toxin’, Onderstepoort Journal of Veterinary Research, vol. 42, no. 3, pp. 91-98. en_ZA
dc.identifier.issn 0330-2465
dc.language.iso en en_ZA
dc.publisher Published by The Government Printer, Pretoria en_ZA
dc.rights ©1975 ARC-Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). ©2016 University of Pretoria. Department of Library Services (digital). en_ZA
dc.subject Veterinary medicine en_ZA
dc.subject.lcsh Veterinary medicine -- South Africa
dc.title The partial purification of Clostridium perfringens Beta toxin en_ZA
dc.type Article en_ZA

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