Additional file 1: Figure S1. Phylograms of C. siamense s. lat. resulted
from the RAxML analyses based on the seven single loci, five-locus and
eight-locus alignments, only bootstrap support value > 50 % are shown.
Each isolate was marked with the’clade’ number that corresponds to the
ApMat tree (Fig. 1).
Additional file 2: Table S1. Pairwise homoplasy index (PHI) of paired
clades in C. siamense s. lat.
Additional file 3: Figure S2. Super-network obtained from the
combined analyses of single-gene ML trees (ApMat, CAL, GAPDH, GS, ITS,
TUB2). The scale indicates the mean distance obtained from the analysis
of single-gene trees.
Additional file 4: Figure S3. Ultrametric gene genealogy and clusters
recognized by the single-threshold method of GMYC (Coalescent model).
Putative species clusters are indicated using transitions between blackcolored
to red-colored branches. The inter- and intraspecific portions of
the tree are divided with a vertical line.
Additional file 5: Figure S4. Ultrametric gene genealogy and clusters
recognized by the multi-threshold method of GMYC (Coalescent model).
Putative species clusters are indicated using transitions between blackcolored
to red-colored branches. The inter- and intraspecific portions of
the tree are divided with a vertical line.
Additional file 6: Figure S5. Results of the PTP analysis based on the BI
and ML topologies. Putative species clusters are indicated using
transitions between blue-colored to red-colored branches.
Additional file 7: Figure S6. Development of sexual structures through
the interaction of isolates LC2937 × LC2875. a. mature perithecia. b, c.
Asci and ascospores. Scale bars: b–c = 10 μm. (
Additional file 8: Table S2. Sexual compatibility between isolates of C.
siamense s. lat.
Additional file 9: Figure S7. Dendrogram resulted from the
hierarchical clustering analysis with the Ward’s method showing the
distribution of mean spore lengths and widths of isolatesof C. siamense s.
lat.
Additional file 10: Figure S8. Discordance between genes trees of
ApMat (left) and 5-locus (CAL, GAPDH, GS, ITS, TUB2) (right) constructed
with a maximum likelihood analysis by running RAxML v.7.0.3. The RAxML
bootstrap support values (ML, >50) and Bayesian posterior probabilities
(PP, >0.95) are displayed at the nodes (ML/PP). Ex-type cultures of described
species within in C. siamense s. lat. indicated with red color.
Additional file 11: Table S3. Details of isolates included in the
phylogenetic analyses and species delimitation.
Additional file 12: Table S4 Primers used in this study, with sequences
and sources.