A sequential co-extraction method for DNA, RNA and protein recovery from soil for future system-based approaches
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Date
Authors
Gunnigle, Eoin
Ramond, Jean-Baptiste
Frossard, Aline
Seely, Mary
Cowan, Don A.
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
A co-extraction protocol that sequentially isolates core biopolymer fractions (DNA, RNA, protein) from edaphic
microbial communities is presented. In order to confirm compatibility with downstream analyses, bacterial
T-RFLP profiles were generated from the DNA- and RNA-derived fractions of an arid-based soil, with
metaproteomics undertaken on the corresponding protein fraction.
Description
Keywords
Co-extraction, Nucleic acids, Protein, T-RFLP, Metaproteomics
Sustainable Development Goals
Citation
Gunnigle, E, Ramond, JB, Frossard, A, Seeley, M & Cowan, D 2014, 'A sequential co-extraction method for DNA, RNA and protein recovery from soil for future system-based approaches', Journal of Microbiological Methods, vol. 103, pp. 118-123.