A sequential co-extraction method for DNA, RNA and protein recovery from soil for future system-based approaches
| dc.contributor.author | Gunnigle, Eoin | |
| dc.contributor.author | Ramond, Jean-Baptiste | |
| dc.contributor.author | Frossard, Aline | |
| dc.contributor.author | Seely, Mary | |
| dc.contributor.author | Cowan, Don A. | |
| dc.contributor.email | don.cowan@up.ac.za | en_ZA |
| dc.date.accessioned | 2016-05-31T05:06:09Z | |
| dc.date.available | 2016-05-31T05:06:09Z | |
| dc.date.issued | 2014-08 | |
| dc.description.abstract | A co-extraction protocol that sequentially isolates core biopolymer fractions (DNA, RNA, protein) from edaphic microbial communities is presented. In order to confirm compatibility with downstream analyses, bacterial T-RFLP profiles were generated from the DNA- and RNA-derived fractions of an arid-based soil, with metaproteomics undertaken on the corresponding protein fraction. | en_ZA |
| dc.description.department | Genetics | en_ZA |
| dc.description.librarian | hb2016 | en_ZA |
| dc.description.sponsorship | National Research Foundation Grant no. 81779 (South Africa). | en_ZA |
| dc.description.uri | http://www.elsevier.com/ locate/jmicmeth | en_ZA |
| dc.identifier.citation | Gunnigle, E, Ramond, JB, Frossard, A, Seeley, M & Cowan, D 2014, 'A sequential co-extraction method for DNA, RNA and protein recovery from soil for future system-based approaches', Journal of Microbiological Methods, vol. 103, pp. 118-123. | en_ZA |
| dc.identifier.issn | 0167-7012 (print) | |
| dc.identifier.issn | 1872-8359 (online) | |
| dc.identifier.other | 10.1016/j.mimet.2014.06.004 | |
| dc.identifier.uri | http://hdl.handle.net/2263/52790 | |
| dc.language.iso | en | en_ZA |
| dc.publisher | Elsevier | en_ZA |
| dc.rights | © 2014 Elsevier B.V. All rights reserved. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Journal of Microbiological Methods. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Microbiological Methods, vol. 103, pp. 118-123, 2014. doi : 10.1016/j.mimet.2014.06.004. | en_ZA |
| dc.subject | Co-extraction | en_ZA |
| dc.subject | Nucleic acids | en_ZA |
| dc.subject | Protein | en_ZA |
| dc.subject | T-RFLP | en_ZA |
| dc.subject | Metaproteomics | en_ZA |
| dc.title | A sequential co-extraction method for DNA, RNA and protein recovery from soil for future system-based approaches | en_ZA |
| dc.type | Postprint Article | en_ZA |