How methylglyoxal kills bacteria : an ultrastructural study

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dc.contributor.author Rabie, E.
dc.contributor.author Serem, June Cheptoo
dc.contributor.author Oberholzer, Hester Magdalena
dc.contributor.author Gaspar, A.R.M. (Anabella Regina Marques)
dc.contributor.author Bester, Megan Jean
dc.date.accessioned 2016-04-26T08:09:29Z
dc.date.issued 2016-03
dc.description.abstract Antibacterial activity of honey is due to the presence of methylglyoxal (MGO), H2O2, bee defensin as well as polyphenols. High MGO levels in manuka honey are the main source of antibacterial activity. Manuka honey has been reported to reduce the swarming and swimming motility of Pseudomonas aeruginosa due to de-flagellation. Due to the complexity of honey it is unknown if this effect is directly due to MGO. In this ultrastructural investigation the effects of MGO on the morphology of bacteria and specifically the structure of fimbriae and flagella were investigated. MGO effectively inhibited Gram positive (Bacillus subtilis; MIC 0.8 mM and Staphylococcus aureus; MIC 1.2 mM) and Gram negative (P. aeruginosa; MIC 1.0 mM and Escherichia coli; MIC 1.2 mM) bacteria growth. The ultrastructural effects of 0.5, 1.0 and 2 mM MGO on B. substilis and E. coli morphology was then evaluated. At 0.5 mM MGO, bacteria structure was unaltered. For both bacteria at 1 mM MGO fewer fimbriae were present and the flagella were less or absent. Identified structures appeared stunted and fragile. At 2 mM MGO fimbriae and flagella were absent while the bacteria were rounded with shrinkage and loss of membrane integrity. Antibacterial MGO causes alterations in the structure of bacterial fimbriae and flagella which would limit bacteria adherence and motility. en_ZA
dc.description.department Anatomy en_ZA
dc.description.department Biochemistry en_ZA
dc.description.embargo 2017-03-31
dc.description.librarian hb2016 en_ZA
dc.description.uri http://www.tandfonline.com/loi/iusp20 en_ZA
dc.identifier.citation Rabie, E, Serem, JC, Oberholzer, HM, Gaspar, ARM &, Bester, MJ 2016, 'How methylglyoxal kills bacteria : an ultrastructural study', Ultrastructural Pathology, vol. 40, no. 2, pp. 107-111. en_ZA
dc.identifier.issn 0191-3123 (print)
dc.identifier.issn 1521-0758 (online)
dc.identifier.other 10.3109/01913123.2016.1154914
dc.identifier.uri http://hdl.handle.net/2263/52156
dc.language.iso en en_ZA
dc.publisher Taylor and Francis en_ZA
dc.rights © Informa Healthcare. This is an electronic version of an article published in Ultrastructural Pathology, vol. 40, no. 2, pp. 107-111, 2016. doi : 10.3109/01913123.2016.1154914. Ultrastructural Pathology is available online at : http://www.tandfonline.com/loi/iusp20. en_ZA
dc.subject Antibacterial en_ZA
dc.subject Flagella en_ZA
dc.subject Fimbriae en_ZA
dc.subject Methylglyoxal (MGO) en_ZA
dc.title How methylglyoxal kills bacteria : an ultrastructural study en_ZA
dc.type Postprint Article en_ZA


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