Molecular genomics resource for the parasitic nematode Spirocerca lupi : identification of 149 microsatellite loci using FIASCO and next generation sequencing

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dc.contributor.author Reid, Kerry
dc.contributor.author Mitha, Janishtha R.
dc.contributor.author Greeff, Jaco M. (Jacobus Maree)
dc.contributor.author De Waal, P.J. (Pamela Jean)
dc.date.accessioned 2016-04-21T08:05:39Z
dc.date.issued 2015-09
dc.description.abstract Understanding genetic diversity and movement patterns in parasitic organisms is paramount to establish control and management strategies. In this study we developed a microsatellite resource as well as a diagnostic multiplex for the cosmopolitan parasitic nematode Spirocerca lupi, known to cause spirocercosis in canids. A combination of microsatellite enrichment and 454 sequencing was used to identify 149 unique microsatellite loci in S. lupi. Twenty loci were characterized further in two sampling sites in South Africa, with 10 loci identified as polymorphic (allele ranges from 4 to 17). These loci were designed into a single diagnostic multiplex suitable for species identification and population genetics studies. The markers were also successful in cross-species amplification in Cylicospirura felineus, Philonema oncorhynchi and Gongylonema pulchrum. Our resource provides a large set of candidate loci for a number of nematode studies as well as loci suitable for diversity and population genetics studies of S. lupi within the South African context as well as globally. en_ZA
dc.description.department Genetics en_ZA
dc.description.embargo 2016-09-30
dc.description.librarian hb2016 en_ZA
dc.description.sponsorship Animal and Zoonotic Diseases (AZD) Institutional Research Theme (IRT) at the University of Pretoria (UP).National Research Foundation of South Africa : UID78566 (NRF RISP grant for the ABI3500) and UID 80678. en_ZA
dc.description.uri http://www.journals.elsevier.com/molecular-and-biochemical-parasitology/ en_ZA
dc.identifier.citation Reid, K, Mitha, JR, Greeff, JM & De Waal, PJ 2015, 'Molecular genomics resource for the parasitic nematode Spirocerca lupi :identification of 149 microsatellite loci using FIASCO and next generation sequencing', Molecular and Biochemical Parasitology, vol. 2013, no. 1-2, pp. 1-4. en_ZA
dc.identifier.issn 0166-6851 (print)
dc.identifier.issn 1872-9428 (online)
dc.identifier.other 10.1016/j.molbiopara.2015.09.008
dc.identifier.uri http://hdl.handle.net/2263/52096
dc.language.iso en en_ZA
dc.publisher Elsevier en_ZA
dc.rights © 2015 Elsevier B.V. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Molecular and Biochemical Parasitology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Molecular and Biochemical Parasitology, vol. 203, no. 1-2, pp.1-4, 2015. doi : 10.1016/j.molbiopara.2015.09.008. en_ZA
dc.subject 454 Sequencing en_ZA
dc.subject Canids en_ZA
dc.subject Cross-species amplification en_ZA
dc.subject Microsatellites en_ZA
dc.subject Parasitic nematode en_ZA
dc.subject South Africa en_ZA
dc.title Molecular genomics resource for the parasitic nematode Spirocerca lupi : identification of 149 microsatellite loci using FIASCO and next generation sequencing en_ZA
dc.type Postprint Article en_ZA


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