Molecular genomics resource for the parasitic nematode Spirocerca lupi : identification of 149 microsatellite loci using FIASCO and next generation sequencing

dc.contributor.authorReid, Kerry
dc.contributor.authorMitha, Janishtha R.
dc.contributor.authorGreeff, Jacobus Maree
dc.contributor.authorDe Waal, Pamela Jean
dc.contributor.emailpam.dewaal@up.ac.zaen_ZA
dc.date.accessioned2016-04-21T08:05:39Z
dc.date.issued2015-09
dc.description.abstractUnderstanding genetic diversity and movement patterns in parasitic organisms is paramount to establish control and management strategies. In this study we developed a microsatellite resource as well as a diagnostic multiplex for the cosmopolitan parasitic nematode Spirocerca lupi, known to cause spirocercosis in canids. A combination of microsatellite enrichment and 454 sequencing was used to identify 149 unique microsatellite loci in S. lupi. Twenty loci were characterized further in two sampling sites in South Africa, with 10 loci identified as polymorphic (allele ranges from 4 to 17). These loci were designed into a single diagnostic multiplex suitable for species identification and population genetics studies. The markers were also successful in cross-species amplification in Cylicospirura felineus, Philonema oncorhynchi and Gongylonema pulchrum. Our resource provides a large set of candidate loci for a number of nematode studies as well as loci suitable for diversity and population genetics studies of S. lupi within the South African context as well as globally.en_ZA
dc.description.departmentGeneticsen_ZA
dc.description.embargo2016-09-30
dc.description.librarianhb2016en_ZA
dc.description.sponsorshipAnimal and Zoonotic Diseases (AZD) Institutional Research Theme (IRT) at the University of Pretoria (UP).National Research Foundation of South Africa : UID78566 (NRF RISP grant for the ABI3500) and UID 80678.en_ZA
dc.description.urihttp://www.journals.elsevier.com/molecular-and-biochemical-parasitology/en_ZA
dc.identifier.citationReid, K, Mitha, JR, Greeff, JM & De Waal, PJ 2015, 'Molecular genomics resource for the parasitic nematode Spirocerca lupi :identification of 149 microsatellite loci using FIASCO and next generation sequencing', Molecular and Biochemical Parasitology, vol. 2013, no. 1-2, pp. 1-4.en_ZA
dc.identifier.issn0166-6851 (print)
dc.identifier.issn1872-9428 (online)
dc.identifier.other10.1016/j.molbiopara.2015.09.008
dc.identifier.urihttp://hdl.handle.net/2263/52096
dc.language.isoenen_ZA
dc.publisherElsevieren_ZA
dc.rights© 2015 Elsevier B.V. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Molecular and Biochemical Parasitology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Molecular and Biochemical Parasitology, vol. 203, no. 1-2, pp.1-4, 2015. doi : 10.1016/j.molbiopara.2015.09.008.en_ZA
dc.subject454 Sequencingen_ZA
dc.subjectCanidsen_ZA
dc.subjectCross-species amplificationen_ZA
dc.subjectMicrosatellitesen_ZA
dc.subjectParasitic nematodeen_ZA
dc.subjectSouth Africaen_ZA
dc.titleMolecular genomics resource for the parasitic nematode Spirocerca lupi : identification of 149 microsatellite loci using FIASCO and next generation sequencingen_ZA
dc.typePostprint Articleen_ZA

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